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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (icaB) CSB-YP750066SKV
CSB-EP750066SKV
CSB-BP750066SKV
CSB-MP750066SKV
CSB-EP750066SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginine deiminase (arcA) CSB-YP750067SKV
CSB-EP750067SKV
CSB-BP750067SKV
CSB-MP750067SKV
CSB-EP750067SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP750068SKV
CSB-EP750068SKV
CSB-BP750068SKV
CSB-MP750068SKV
CSB-EP750068SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable transglycosylase isaA (isaA) CSB-YP750069SKV
CSB-EP750069SKV
CSB-BP750069SKV
CSB-MP750069SKV
CSB-EP750069SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Copper-exporting P-type ATPase A (copA), partial CSB-YP750070SKV
CSB-EP750070SKV
CSB-BP750070SKV
CSB-MP750070SKV
CSB-EP750070SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-lactate dehydrogenase (ldhD) CSB-YP750071SKV
CSB-EP750071SKV
CSB-BP750071SKV
CSB-MP750071SKV
CSB-EP750071SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-carboxyhexanoate--CoA ligase (bioW) CSB-YP750072SKV
CSB-EP750072SKV
CSB-BP750072SKV
CSB-MP750072SKV
CSB-EP750072SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ferredoxin--NADP reductase (SAR2461) CSB-YP750073SKV
CSB-EP750073SKV
CSB-BP750073SKV
CSB-MP750073SKV
CSB-EP750073SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Membrane-associated protein TcaA (tcaA), partial CSB-YP750074SKV
CSB-EP750074SKV
CSB-BP750074SKV
CSB-MP750074SKV
CSB-EP750074SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lysostaphin resistance protein A (lyrA), partial CSB-YP750075SKV
CSB-EP750075SKV
CSB-BP750075SKV
CSB-MP750075SKV
CSB-EP750075SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Staphylococcal secretory antigen ssaA2 (ssaA2) CSB-YP750076SKV
CSB-EP750076SKV
CSB-BP750076SKV
CSB-MP750076SKV
CSB-EP750076SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease accessory protein UreG (ureG) CSB-YP750077SKV
CSB-EP750077SKV
CSB-BP750077SKV
CSB-MP750077SKV
CSB-EP750077SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator sarV (sarV) CSB-YP750078SKV
CSB-EP750078SKV
CSB-BP750078SKV
CSB-MP750078SKV
CSB-EP750078SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L4 (rplD) CSB-YP750079SKV
CSB-EP750079SKV
CSB-BP750079SKV
CSB-MP750079SKV
CSB-EP750079SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L16 (rplP) CSB-YP750080SKV
CSB-EP750080SKV
CSB-BP750080SKV
CSB-MP750080SKV
CSB-EP750080SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L5 (rplE) CSB-YP750081SKV
CSB-EP750081SKV
CSB-BP750081SKV
CSB-MP750081SKV
CSB-EP750081SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S5 (rpsE) CSB-YP750082SKV
CSB-EP750082SKV
CSB-BP750082SKV
CSB-MP750082SKV
CSB-EP750082SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Adenylate kinase (adk) CSB-YP750083SKV
CSB-EP750083SKV
CSB-BP750083SKV
CSB-MP750083SKV
CSB-EP750083SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA pseudouridine synthase A (truA) CSB-YP750084SKV
CSB-EP750084SKV
CSB-BP750084SKV
CSB-MP750084SKV
CSB-EP750084SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lactose phosphotransferase system repressor (lacR) CSB-YP750085SKV
CSB-EP750085SKV
CSB-BP750085SKV
CSB-MP750085SKV
CSB-EP750085SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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