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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii ADIPOR-like receptor IZH1 (IZH1), partial CSB-YP748344DOT1
CSB-EP748344DOT1
CSB-BP748344DOT1
CSB-MP748344DOT1
CSB-EP748344DOT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable kinetochore protein NDC80 (NDC80), partial CSB-YP748345DOT
CSB-EP748345DOT
CSB-BP748345DOT
CSB-MP748345DOT
CSB-EP748345DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Vacuolar-sorting protein SNF7 (SNF7) CSB-YP748346DOT
CSB-EP748346DOT
CSB-BP748346DOT
CSB-MP748346DOT
CSB-EP748346DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Increased recombination centers protein 19 (IRC19) CSB-YP748347DOT
CSB-EP748347DOT
CSB-BP748347DOT
CSB-MP748347DOT
CSB-EP748347DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein SYM1 (SYM1), partial CSB-YP748348DOT1
CSB-EP748348DOT1
CSB-BP748348DOT1
CSB-MP748348DOT1
CSB-EP748348DOT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribosome-releasing factor 2, mitochondrial (MEF2), partial CSB-YP748349DOT
CSB-EP748349DOT
CSB-BP748349DOT
CSB-MP748349DOT
CSB-EP748349DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal protein L44 (RPL44) CSB-YP748350DOT
CSB-EP748350DOT
CSB-BP748350DOT
CSB-MP748350DOT
CSB-EP748350DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ubiquitin carboxyl-terminal hydrolase 4 (DOA4), partial CSB-YP748351DOT
CSB-EP748351DOT
CSB-BP748351DOT
CSB-MP748351DOT
CSB-EP748351DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (PRP5), partial CSB-YP748352DOT
CSB-EP748352DOT
CSB-BP748352DOT
CSB-MP748352DOT
CSB-EP748352DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable endonuclease LCL3 (LCL3), partial CSB-YP748353DOT1
CSB-EP748353DOT1
CSB-BP748353DOT1
CSB-MP748353DOT1
CSB-EP748353DOT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Spindle assembly checkpoint kinase (IPL1) CSB-YP748354DOT
CSB-EP748354DOT
CSB-BP748354DOT
CSB-MP748354DOT
CSB-EP748354DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Lon protease homolog 2, peroxisomal (AFL121W), partial CSB-YP748355DOT
CSB-EP748355DOT
CSB-BP748355DOT
CSB-MP748355DOT
CSB-EP748355DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein ROT1 (ROT1), partial CSB-YP748356DOT1
CSB-EP748356DOT1
CSB-BP748356DOT1
CSB-MP748356DOT1
CSB-EP748356DOT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribosome biogenesis protein ALB1 (ALB1) CSB-YP748357DOT
CSB-EP748357DOT
CSB-BP748357DOT
CSB-MP748357DOT
CSB-EP748357DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein translation factor SUI1 (SUI1A) CSB-YP748358DOT
CSB-EP748358DOT
CSB-BP748358DOT
CSB-MP748358DOT
CSB-EP748358DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Actin cytoskeleton-regulatory complex protein END3 (END3) CSB-YP748359DOT
CSB-EP748359DOT
CSB-BP748359DOT
CSB-MP748359DOT
CSB-EP748359DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Regulator of rDNA transcription 14 (RRT14) CSB-YP748360DOT
CSB-EP748360DOT
CSB-BP748360DOT
CSB-MP748360DOT
CSB-EP748360DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii pH-response regulator protein palA/RIM20 (RIM20), partial CSB-YP748361DOT
CSB-EP748361DOT
CSB-BP748361DOT
CSB-MP748361DOT
CSB-EP748361DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 13 (SSN2), partial CSB-YP748362DOT
CSB-EP748362DOT
CSB-BP748362DOT
CSB-MP748362DOT
CSB-EP748362DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Enolase (ENO) CSB-YP748363DOT
CSB-EP748363DOT
CSB-BP748363DOT
CSB-MP748363DOT
CSB-EP748363DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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