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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sulfurreducens Flagellar assembly factor FliW (fliW) CSB-YP748244GBK
CSB-EP748244GBK
CSB-BP748244GBK
CSB-MP748244GBK
CSB-EP748244GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L13 (rplM) CSB-YP748245GBK
CSB-EP748245GBK
CSB-BP748245GBK
CSB-MP748245GBK
CSB-EP748245GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S7 (rpsG) CSB-YP748246GBK
CSB-EP748246GBK
CSB-BP748246GBK
CSB-MP748246GBK
CSB-EP748246GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S17 (rpsQ) CSB-YP748247GBK
CSB-EP748247GBK
CSB-BP748247GBK
CSB-MP748247GBK
CSB-EP748247GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S5 (rpsE) CSB-YP748248GBK
CSB-EP748248GBK
CSB-BP748248GBK
CSB-MP748248GBK
CSB-EP748248GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UPF0324 membrane protein GSU2818 (GSU2818), partial CSB-YP748249GBK
CSB-EP748249GBK
CSB-BP748249GBK
CSB-MP748249GBK
CSB-EP748249GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Malonyl-CoA O-methyltransferase BioC (bioC) CSB-YP748250GBK
CSB-EP748250GBK
CSB-BP748250GBK
CSB-MP748250GBK
CSB-EP748250GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Elongation factor G 1 (fusA1), partial CSB-YP748251GBK
CSB-EP748251GBK
CSB-BP748251GBK
CSB-MP748251GBK
CSB-EP748251GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Tryptophan synthase alpha chain (trpA) CSB-YP748252GBK
CSB-EP748252GBK
CSB-BP748252GBK
CSB-MP748252GBK
CSB-EP748252GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Lipid-A-disaccharide synthase (lpxB) CSB-YP748253GBK
CSB-EP748253GBK
CSB-BP748253GBK
CSB-MP748253GBK
CSB-EP748253GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S20 (rpsT) CSB-YP748254GBK
CSB-EP748254GBK
CSB-BP748254GBK
CSB-MP748254GBK
CSB-EP748254GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP748255GBK
CSB-EP748255GBK
CSB-BP748255GBK
CSB-MP748255GBK
CSB-EP748255GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens tRNA dimethylallyltransferase (miaA) CSB-YP748256GBK
CSB-EP748256GBK
CSB-BP748256GBK
CSB-MP748256GBK
CSB-EP748256GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP748257GBK
CSB-EP748257GBK
CSB-BP748257GBK
CSB-MP748257GBK
CSB-EP748257GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens tRNA (Ile)-lysidine synthase (tilS) CSB-YP748258GBK
CSB-EP748258GBK
CSB-BP748258GBK
CSB-MP748258GBK
CSB-EP748258GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP748259GBK
CSB-EP748259GBK
CSB-BP748259GBK
CSB-MP748259GBK
CSB-EP748259GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens SsrA-binding protein (smpB) CSB-YP748260GBK
CSB-EP748260GBK
CSB-BP748260GBK
CSB-MP748260GBK
CSB-EP748260GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Acyl carrier protein (acpP) CSB-YP748261GBK
CSB-EP748261GBK
CSB-BP748261GBK
CSB-MP748261GBK
CSB-EP748261GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Biotin synthase (bioB) CSB-YP748262GBK
CSB-EP748262GBK
CSB-BP748262GBK
CSB-MP748262GBK
CSB-EP748262GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L35 (rpmI) CSB-YP748263GBK
CSB-EP748263GBK
CSB-BP748263GBK
CSB-MP748263GBK
CSB-EP748263GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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