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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Calycanthus floridus var. glaucus Photosystem II reaction center protein M (psbM), partial CSB-YP748684CBM
CSB-EP748684CBM
CSB-BP748684CBM
CSB-MP748684CBM
CSB-EP748684CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aotus nigriceps Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP748685AFAL1
CSB-EP748685AFAL1
CSB-BP748685AFAL1
CSB-MP748685AFAL1
CSB-EP748685AFAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taxus cuspidata Maturase K (matK), partial CSB-YP748686TCAC
CSB-EP748686TCAC
CSB-BP748686TCAC
CSB-MP748686TCAC
CSB-EP748686TCAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nymphaea odorata Cytochrome b559 subunit beta (psbF), partial CSB-YP748687NIE
CSB-EP748687NIE
CSB-BP748687NIE
CSB-MP748687NIE
CSB-EP748687NIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tamias dorsalis Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP748688TCAI1
CSB-EP748688TCAI1
CSB-BP748688TCAI1
CSB-MP748688TCAI1
CSB-EP748688TCAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dioscorea bulbifera Photosystem II reaction center protein T (psbT), partial CSB-YP748689DCAL
CSB-EP748689DCAL
CSB-BP748689DCAL
CSB-MP748689DCAL
CSB-EP748689DCAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acorus calamus Protein psbN (psbN), partial CSB-YP748690ACE
CSB-EP748690ACE
CSB-BP748690ACE
CSB-MP748690ACE
CSB-EP748690ACE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein L (psbL), partial CSB-YP748691LCAX
CSB-EP748691LCAX
CSB-BP748691LCAX
CSB-MP748691LCAX
CSB-EP748691LCAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cabomba caroliniana Photosystem II reaction center protein L (psbL), partial CSB-YP748692CAA
CSB-EP748692CAA
CSB-BP748692CAA
CSB-MP748692CAA
CSB-EP748692CAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Moschus chrysogaster Cytochrome b (MT-CYB), partial CSB-YP748693MFAA
CSB-EP748693MFAA
CSB-BP748693MFAA
CSB-MP748693MFAA
CSB-EP748693MFAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trillium erectum Maturase K (matK), partial CSB-YP748694TCAJ
CSB-EP748694TCAJ
CSB-BP748694TCAJ
CSB-MP748694TCAJ
CSB-EP748694TCAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca tonkeana Twist-related protein 1 (TWIST1) CSB-YP748695MAAO
CSB-EP748695MAAO
CSB-BP748695MAAO
CSB-MP748695MAAO
CSB-EP748695MAAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monophyllus redmani Sperm protamine P1 (PRM1) CSB-YP748696MFAR
CSB-EP748696MFAR
CSB-BP748696MFAR
CSB-MP748696MFAR
CSB-EP748696MFAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fuscata fuscata Cystic fibrosis transmembrane conductance regulator (CFTR), partial CSB-YP748697MMX
CSB-EP748697MMX
CSB-BP748697MMX
CSB-MP748697MMX
CSB-EP748697MMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Beta-1,3-galactosyltransferase 1 (B3GALT1), partial CSB-YP748698GGZ
CSB-EP748698GGZ
CSB-BP748698GGZ
CSB-MP748698GGZ
CSB-EP748698GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein B0361.3 (B0361.3), partial CSB-YP748699CXY
CSB-EP748699CXY
CSB-BP748699CXY
CSB-MP748699CXY
CSB-EP748699CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster PTB domain-containing adapter protein ced-6 (ced-6), partial CSB-YP748700DLU
CSB-EP748700DLU
CSB-BP748700DLU
CSB-MP748700DLU
CSB-EP748700DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Mannose-1-phosphate guanyltransferase beta (CG1129) CSB-YP748701DLU
CSB-EP748701DLU
CSB-BP748701DLU
CSB-MP748701DLU
CSB-EP748701DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Zinc finger protein GLIS2 homolog (sug) CSB-YP748702DLU
CSB-EP748702DLU
CSB-BP748702DLU
CSB-MP748702DLU
CSB-EP748702DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GalTA), partial CSB-YP748703DLU
CSB-EP748703DLU
CSB-BP748703DLU
CSB-MP748703DLU
CSB-EP748703DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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