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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris Anthranilate phosphoribosyltransferase (trpD) CSB-YP749284EYQ
CSB-EP749284EYQ
CSB-BP749284EYQ
CSB-MP749284EYQ
CSB-EP749284EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP749285EYQ
CSB-EP749285EYQ
CSB-BP749285EYQ
CSB-MP749285EYQ
CSB-EP749285EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris CinA-like protein (PMM0257) CSB-YP749286EYQ
CSB-EP749286EYQ
CSB-BP749286EYQ
CSB-MP749286EYQ
CSB-EP749286EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP749287EYO
CSB-EP749287EYO
CSB-BP749287EYO
CSB-MP749287EYO
CSB-EP749287EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit I (ndhI) CSB-YP749288EYO
CSB-EP749288EYO
CSB-BP749288EYO
CSB-MP749288EYO
CSB-EP749288EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L36 (rpmJ) CSB-YP749289EYO
CSB-EP749289EYO
CSB-BP749289EYO
CSB-MP749289EYO
CSB-EP749289EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP749290EYO1
CSB-EP749290EYO1
CSB-BP749290EYO1
CSB-MP749290EYO1
CSB-EP749290EYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamate--tRNA ligase (gltX) CSB-YP749291EYO
CSB-EP749291EYO
CSB-BP749291EYO
CSB-MP749291EYO
CSB-EP749291EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S18 (rpsR) CSB-YP749292EYO
CSB-EP749292EYO
CSB-BP749292EYO
CSB-MP749292EYO
CSB-EP749292EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP749293EYO
CSB-EP749293EYO
CSB-BP749293EYO
CSB-MP749293EYO
CSB-EP749293EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Indole-3-glycerol phosphate synthase (trpC) CSB-YP749294EYO
CSB-EP749294EYO
CSB-BP749294EYO
CSB-MP749294EYO
CSB-EP749294EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus D-fructose 1,6-bisphosphatase class 2/sedoheptulose 1,7-bisphosphatase (Pro_0840) CSB-YP749295EYP
CSB-EP749295EYP
CSB-BP749295EYP
CSB-MP749295EYP
CSB-EP749295EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyltransferase papA1 (papA1), partial CSB-YP749296MVH
CSB-EP749296MVH
CSB-BP749296MVH
CSB-MP749296MVH
CSB-EP749296MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-isopropylmalate synthase (leuA), partial CSB-YP749297MVH
CSB-EP749297MVH
CSB-BP749297MVH
CSB-MP749297MVH
CSB-EP749297MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trehalose-phosphate synthase (otsA) CSB-YP749298MVH
CSB-EP749298MVH
CSB-BP749298MVH
CSB-MP749298MVH
CSB-EP749298MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maf-like protein Mb3310 (Mb3310) CSB-YP749299MVH
CSB-EP749299MVH
CSB-BP749299MVH
CSB-MP749299MVH
CSB-EP749299MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP749300MVH
CSB-EP749300MVH
CSB-BP749300MVH
CSB-MP749300MVH
CSB-EP749300MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP749301MVH
CSB-EP749301MVH
CSB-BP749301MVH
CSB-MP749301MVH
CSB-EP749301MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trans-acting enoyl reductase (Mb2977) CSB-YP749302MVH
CSB-EP749302MVH
CSB-BP749302MVH
CSB-MP749302MVH
CSB-EP749302MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsE (ppsE), partial CSB-YP749303MVH
CSB-EP749303MVH
CSB-BP749303MVH
CSB-MP749303MVH
CSB-EP749303MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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