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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Autoinducer 2 kinase LsrK (lsrK), partial CSB-YP748944PIJ
CSB-EP748944PIJ
CSB-BP748944PIJ
CSB-MP748944PIJ
CSB-EP748944PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP748945PIJ1
CSB-EP748945PIJ1
CSB-BP748945PIJ1
CSB-MP748945PIJ1
CSB-EP748945PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP748946PIJ
CSB-EP748946PIJ
CSB-BP748946PIJ
CSB-MP748946PIJ
CSB-EP748946PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0352 protein plu2871 (plu2871) CSB-YP748947PIJ
CSB-EP748947PIJ
CSB-BP748947PIJ
CSB-MP748947PIJ
CSB-EP748947PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Phosphate acyltransferase (plsX) CSB-YP748948PIJ
CSB-EP748948PIJ
CSB-BP748948PIJ
CSB-MP748948PIJ
CSB-EP748948PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0339 protein plu2779 (plu2779) CSB-YP748949PIJ
CSB-EP748949PIJ
CSB-BP748949PIJ
CSB-MP748949PIJ
CSB-EP748949PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP748950PIJ
CSB-EP748950PIJ
CSB-BP748950PIJ
CSB-MP748950PIJ
CSB-EP748950PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP748951PIJ
CSB-EP748951PIJ
CSB-BP748951PIJ
CSB-MP748951PIJ
CSB-EP748951PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ProP effector (proQ) CSB-YP748952PIJ
CSB-EP748952PIJ
CSB-BP748952PIJ
CSB-MP748952PIJ
CSB-EP748952PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP748953PIJ
CSB-EP748953PIJ
CSB-BP748953PIJ
CSB-MP748953PIJ
CSB-EP748953PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP748954PIJ1
CSB-EP748954PIJ1
CSB-BP748954PIJ1
CSB-MP748954PIJ1
CSB-EP748954PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii HTH-type transcriptional repressor PurR (purR) CSB-YP748955PIJ
CSB-EP748955PIJ
CSB-BP748955PIJ
CSB-MP748955PIJ
CSB-EP748955PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP748956PIJ
CSB-EP748956PIJ
CSB-BP748956PIJ
CSB-MP748956PIJ
CSB-EP748956PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0263 protein plu2488 (plu2488) CSB-YP748957PIJ
CSB-EP748957PIJ
CSB-BP748957PIJ
CSB-MP748957PIJ
CSB-EP748957PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP748958PIJ
CSB-EP748958PIJ
CSB-BP748958PIJ
CSB-MP748958PIJ
CSB-EP748958PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Xylose isomerase (xylA) CSB-YP748959PIJ
CSB-EP748959PIJ
CSB-BP748959PIJ
CSB-MP748959PIJ
CSB-EP748959PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Pseudouridine-5'-phosphate glycosidase 1 (psuG1) CSB-YP748960PIJ
CSB-EP748960PIJ
CSB-BP748960PIJ
CSB-MP748960PIJ
CSB-EP748960PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0260 protein plu2141 (plu2141) CSB-YP748961PIJ
CSB-EP748961PIJ
CSB-BP748961PIJ
CSB-MP748961PIJ
CSB-EP748961PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP748962PIJ
CSB-EP748962PIJ
CSB-BP748962PIJ
CSB-MP748962PIJ
CSB-EP748962PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Arginine--tRNA ligase (argS), partial CSB-YP748963PIJ
CSB-EP748963PIJ
CSB-BP748963PIJ
CSB-MP748963PIJ
CSB-EP748963PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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