Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP749344SSL
CSB-EP749344SSL
CSB-BP749344SSL
CSB-MP749344SSL
CSB-EP749344SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acetylornithine aminotransferase (argD) CSB-YP749345SSL
CSB-EP749345SSL
CSB-BP749345SSL
CSB-MP749345SSL
CSB-EP749345SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP749346SSL
CSB-EP749346SSL
CSB-BP749346SSL
CSB-MP749346SSL
CSB-EP749346SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Homoserine kinase (thrB) CSB-YP749347SSL
CSB-EP749347SSL
CSB-BP749347SSL
CSB-MP749347SSL
CSB-EP749347SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP749348SSL
CSB-EP749348SSL
CSB-BP749348SSL
CSB-MP749348SSL
CSB-EP749348SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP749349SSL
CSB-EP749349SSL
CSB-BP749349SSL
CSB-MP749349SSL
CSB-EP749349SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S18 (rpsR) CSB-YP749350SSL
CSB-EP749350SSL
CSB-BP749350SSL
CSB-MP749350SSL
CSB-EP749350SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Dihydroxy-acid dehydratase (ilvD), partial CSB-YP749351SSL
CSB-EP749351SSL
CSB-BP749351SSL
CSB-MP749351SSL
CSB-EP749351SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center Psb28 protein (psb28) CSB-YP749352SSL
CSB-EP749352SSL
CSB-BP749352SSL
CSB-MP749352SSL
CSB-EP749352SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP749353SSL
CSB-EP749353SSL
CSB-BP749353SSL
CSB-MP749353SSL
CSB-EP749353SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S15 (rpsO) CSB-YP749354SSL
CSB-EP749354SSL
CSB-BP749354SSL
CSB-MP749354SSL
CSB-EP749354SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP749355SSL
CSB-EP749355SSL
CSB-BP749355SSL
CSB-MP749355SSL
CSB-EP749355SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP749356SSL
CSB-EP749356SSL
CSB-BP749356SSL
CSB-MP749356SSL
CSB-EP749356SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Triosephosphate isomerase (tpiA) CSB-YP749357SSL
CSB-EP749357SSL
CSB-BP749357SSL
CSB-MP749357SSL
CSB-EP749357SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP749358SSL
CSB-EP749358SSL
CSB-BP749358SSL
CSB-MP749358SSL
CSB-EP749358SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Putative Holliday junction resolvase (SYNW0779) CSB-YP749359SSL
CSB-EP749359SSL
CSB-BP749359SSL
CSB-MP749359SSL
CSB-EP749359SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP749360SSL
CSB-EP749360SSL
CSB-BP749360SSL
CSB-MP749360SSL
CSB-EP749360SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acireductone dioxygenase (mtnD) CSB-YP749361SSL
CSB-EP749361SSL
CSB-BP749361SSL
CSB-MP749361SSL
CSB-EP749361SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S20 (rpsT) CSB-YP749362SSL
CSB-EP749362SSL
CSB-BP749362SSL
CSB-MP749362SSL
CSB-EP749362SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Translation initiation factor IF-2 (infB), partial CSB-YP749363SSL
CSB-EP749363SSL
CSB-BP749363SSL
CSB-MP749363SSL
CSB-EP749363SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>