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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP748984PIJ
CSB-EP748984PIJ
CSB-BP748984PIJ
CSB-MP748984PIJ
CSB-EP748984PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Uridylate kinase (pyrH) CSB-YP748985PIJ
CSB-EP748985PIJ
CSB-BP748985PIJ
CSB-MP748985PIJ
CSB-EP748985PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Alkaline proteinase inhibitor (inh) CSB-YP748986PIJ
CSB-EP748986PIJ
CSB-BP748986PIJ
CSB-MP748986PIJ
CSB-EP748986PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii RNA pyrophosphohydrolase (rppH) CSB-YP748987PIJ
CSB-EP748987PIJ
CSB-BP748987PIJ
CSB-MP748987PIJ
CSB-EP748987PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP748988PIJ
CSB-EP748988PIJ
CSB-BP748988PIJ
CSB-MP748988PIJ
CSB-EP748988PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Isoleucine--tRNA ligase (ileS), partial CSB-YP748989PIJ
CSB-EP748989PIJ
CSB-BP748989PIJ
CSB-MP748989PIJ
CSB-EP748989PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Non-canonical purine NTP phosphatase (plu0558) CSB-YP748990PIJ
CSB-EP748990PIJ
CSB-BP748990PIJ
CSB-MP748990PIJ
CSB-EP748990PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii HTH-type transcriptional regulator malT (malT), partial CSB-YP748991PIJ
CSB-EP748991PIJ
CSB-BP748991PIJ
CSB-MP748991PIJ
CSB-EP748991PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 50S ribosomal protein L1 (rplA) CSB-YP748992PIJ
CSB-EP748992PIJ
CSB-BP748992PIJ
CSB-MP748992PIJ
CSB-EP748992PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Protein TusC (tusC) CSB-YP748993PIJ
CSB-EP748993PIJ
CSB-BP748993PIJ
CSB-MP748993PIJ
CSB-EP748993PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Guanylate kinase (gmk) CSB-YP748994PIJ
CSB-EP748994PIJ
CSB-BP748994PIJ
CSB-MP748994PIJ
CSB-EP748994PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Thiosulfate sulfurtransferase glpE (glpE) CSB-YP748995PIJ
CSB-EP748995PIJ
CSB-BP748995PIJ
CSB-MP748995PIJ
CSB-EP748995PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 33 kDa chaperonin (hslO) CSB-YP748996PIJ
CSB-EP748996PIJ
CSB-BP748996PIJ
CSB-MP748996PIJ
CSB-EP748996PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribose import ATP-binding protein RbsA (rbsA), partial CSB-YP748997PIJ
CSB-EP748997PIJ
CSB-BP748997PIJ
CSB-MP748997PIJ
CSB-EP748997PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP synthase subunit b (atpF), partial CSB-YP748998PIJ1
CSB-EP748998PIJ1
CSB-BP748998PIJ1
CSB-MP748998PIJ1
CSB-EP748998PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 50S ribosomal protein L36 (rpmJ) CSB-YP748999PIJ
CSB-EP748999PIJ
CSB-BP748999PIJ
CSB-MP748999PIJ
CSB-EP748999PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Glutamate--tRNA ligase (gltX) CSB-YP749000MWI
CSB-EP749000MWI
CSB-BP749000MWI
CSB-MP749000MWI
CSB-EP749000MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 30S ribosomal protein S6 (rpsF) CSB-YP749001MWI
CSB-EP749001MWI
CSB-BP749001MWI
CSB-MP749001MWI
CSB-EP749001MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP749002MWI
CSB-EP749002MWI
CSB-BP749002MWI
CSB-MP749002MWI
CSB-EP749002MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP749003MWI
CSB-EP749003MWI
CSB-BP749003MWI
CSB-MP749003MWI
CSB-EP749003MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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