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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus maripaludis FO synthase subunit 1 (cofG) CSB-YP750546MSB
CSB-EP750546MSB
CSB-BP750546MSB
CSB-MP750546MSB
CSB-EP750546MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UvrABC system protein B (uvrB), partial CSB-YP750547MSB
CSB-EP750547MSB
CSB-BP750547MSB
CSB-MP750547MSB
CSB-EP750547MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Leucine--tRNA ligase (leuS), partial CSB-YP750548MSB
CSB-EP750548MSB
CSB-BP750548MSB
CSB-MP750548MSB
CSB-EP750548MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ketol-acid reductoisomerase (ilvC) CSB-YP750549MSB
CSB-EP750549MSB
CSB-BP750549MSB
CSB-MP750549MSB
CSB-EP750549MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 1A (eif1a) CSB-YP750550MSB
CSB-EP750550MSB
CSB-BP750550MSB
CSB-MP750550MSB
CSB-EP750550MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Valine--tRNA ligase (valS), partial CSB-YP750551MSB
CSB-EP750551MSB
CSB-BP750551MSB
CSB-MP750551MSB
CSB-EP750551MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S27ae (rps27ae) CSB-YP750552MSB
CSB-EP750552MSB
CSB-BP750552MSB
CSB-MP750552MSB
CSB-EP750552MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis GTP cyclohydrolase III (gch3) CSB-YP750553MSB
CSB-EP750553MSB
CSB-BP750553MSB
CSB-MP750553MSB
CSB-EP750553MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP750554MSB
CSB-EP750554MSB
CSB-BP750554MSB
CSB-MP750554MSB
CSB-EP750554MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L1P (rpl1p) CSB-YP750555MSB
CSB-EP750555MSB
CSB-BP750555MSB
CSB-MP750555MSB
CSB-EP750555MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 7-cyano-7-deazaguanine synthase (queC) CSB-YP750556MSB
CSB-EP750556MSB
CSB-BP750556MSB
CSB-MP750556MSB
CSB-EP750556MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Hypoxanthine/guanine phosphoribosyltransferase (hpt) CSB-YP750557MSB
CSB-EP750557MSB
CSB-BP750557MSB
CSB-MP750557MSB
CSB-EP750557MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP750558MSB
CSB-EP750558MSB
CSB-BP750558MSB
CSB-MP750558MSB
CSB-EP750558MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0425 pyridoxal phosphate-dependent protein MMP0002 (MMP0002) CSB-YP750559MSB
CSB-EP750559MSB
CSB-BP750559MSB
CSB-MP750559MSB
CSB-EP750559MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0324 membrane protein Cgl0015/cg0018 (Cgl0015, cg0018), partial CSB-YP750560DWY
CSB-EP750560DWY
CSB-BP750560DWY
CSB-MP750560DWY
CSB-EP750560DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP750561PAAD
CSB-EP750561PAAD
CSB-BP750561PAAD
CSB-MP750561PAAD
CSB-EP750561PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP750562PAAD
CSB-EP750562PAAD
CSB-BP750562PAAD
CSB-MP750562PAAD
CSB-EP750562PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila UPF0365 protein pc1737 (pc1737), partial CSB-YP750563PAAD1
CSB-EP750563PAAD1
CSB-BP750563PAAD1
CSB-MP750563PAAD1
CSB-EP750563PAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Transaldolase (tal) CSB-YP750564PAAD
CSB-EP750564PAAD
CSB-BP750564PAAD
CSB-MP750564PAAD
CSB-EP750564PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila ATP synthase subunit alpha (atpA), partial CSB-YP750565PAAD
CSB-EP750565PAAD
CSB-BP750565PAAD
CSB-MP750565PAAD
CSB-EP750565PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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