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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gloeobacter violaceus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP749044GCI
CSB-EP749044GCI
CSB-BP749044GCI
CSB-MP749044GCI
CSB-EP749044GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP749045GCI
CSB-EP749045GCI
CSB-BP749045GCI
CSB-MP749045GCI
CSB-EP749045GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Cytochrome b6 (petB), partial CSB-YP749046GCI1
CSB-EP749046GCI1
CSB-BP749046GCI1
CSB-MP749046GCI1
CSB-EP749046GCI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Photosystem II reaction center X protein (psbX), partial CSB-YP749047GCI
CSB-EP749047GCI
CSB-BP749047GCI
CSB-MP749047GCI
CSB-EP749047GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 30S ribosomal protein S2 (rpsB) CSB-YP749048GCI
CSB-EP749048GCI
CSB-BP749048GCI
CSB-MP749048GCI
CSB-EP749048GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP749049GCI
CSB-EP749049GCI
CSB-BP749049GCI
CSB-MP749049GCI
CSB-EP749049GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP749050GCI
CSB-EP749050GCI
CSB-BP749050GCI
CSB-MP749050GCI
CSB-EP749050GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Chromophore lyase CpcT/CpeT 4 (cpcT4) CSB-YP749051GCI
CSB-EP749051GCI
CSB-BP749051GCI
CSB-MP749051GCI
CSB-EP749051GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Glutamate racemase (murI) CSB-YP749052GCI
CSB-EP749052GCI
CSB-BP749052GCI
CSB-MP749052GCI
CSB-EP749052GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Epoxyqueuosine reductase (queG) CSB-YP749053GCI
CSB-EP749053GCI
CSB-BP749053GCI
CSB-MP749053GCI
CSB-EP749053GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Histidinol-phosphate aminotransferase (hisC) CSB-YP749054GCI
CSB-EP749054GCI
CSB-BP749054GCI
CSB-MP749054GCI
CSB-EP749054GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP749055GCI
CSB-EP749055GCI
CSB-BP749055GCI
CSB-MP749055GCI
CSB-EP749055GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Triosephosphate isomerase (tpiA) CSB-YP749056GCI
CSB-EP749056GCI
CSB-BP749056GCI
CSB-MP749056GCI
CSB-EP749056GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus UPF0332 protein glr0978 (glr0978) CSB-YP749057GCI
CSB-EP749057GCI
CSB-BP749057GCI
CSB-MP749057GCI
CSB-EP749057GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Photosystem II reaction center protein J (psbJ), partial CSB-YP749058GCI
CSB-EP749058GCI
CSB-BP749058GCI
CSB-MP749058GCI
CSB-EP749058GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Cysteine--tRNA ligase (cysS) CSB-YP749059GCI
CSB-EP749059GCI
CSB-BP749059GCI
CSB-MP749059GCI
CSB-EP749059GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Phosphate acyltransferase (plsX) CSB-YP749060GCI
CSB-EP749060GCI
CSB-BP749060GCI
CSB-MP749060GCI
CSB-EP749060GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus NAD (P)H-quinone oxidoreductase subunit 3 (ndhC), partial CSB-YP749061GCI1
CSB-EP749061GCI1
CSB-BP749061GCI1
CSB-MP749061GCI1
CSB-EP749061GCI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Nucleoside diphosphate kinase (ndk) CSB-YP749062GCI
CSB-EP749062GCI
CSB-BP749062GCI
CSB-MP749062GCI
CSB-EP749062GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Hemin import ATP-binding protein HmuV (hmuV) CSB-YP749063GCI
CSB-EP749063GCI
CSB-BP749063GCI
CSB-MP749063GCI
CSB-EP749063GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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