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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Keratin, type II cytoskeletal 73 (Krt73) CSB-YP750326RA
CSB-EP750326RA
CSB-BP750326RA
CSB-MP750326RA
CSB-EP750326RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type II cuticular Hb3 (Krt83) CSB-YP750327MO
CSB-EP750327MO
CSB-BP750327MO
CSB-MP750327MO
CSB-EP750327MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sulfotransferase 1C3 (SULT1C3) CSB-YP750328HU
CSB-EP750328HU
CSB-BP750328HU
CSB-MP750328HU
CSB-EP750328HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Nuclear factor interleukin-3-regulated protein (Nfil3) CSB-YP750329RA
CSB-EP750329RA
CSB-BP750329RA
CSB-MP750329RA
CSB-EP750329RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein unc-119 homolog B-B (unc119b-b) CSB-YP750330XBE
CSB-EP750330XBE
CSB-BP750330XBE
CSB-MP750330XBE
CSB-EP750330XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Putative tRNA pseudouridine synthase Pus10 (pus10), partial CSB-YP750331XBE
CSB-EP750331XBE
CSB-BP750331XBE
CSB-MP750331XBE
CSB-EP750331XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein phosphatase 1K, mitochondrial (ppm1k), partial CSB-YP750332XBE
CSB-EP750332XBE
CSB-BP750332XBE
CSB-MP750332XBE
CSB-EP750332XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Peroxisomal membrane protein PEX16 (pex16), partial CSB-YP750333XBE1
CSB-EP750333XBE1
CSB-BP750333XBE1
CSB-MP750333XBE1
CSB-EP750333XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis F-box/LRR-repeat protein 5 (fbxl5), partial CSB-YP750334XBE
CSB-EP750334XBE
CSB-BP750334XBE
CSB-MP750334XBE
CSB-EP750334XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Putative ferric-chelate reductase 1 (frrs1), partial CSB-YP750335XBE1
CSB-EP750335XBE1
CSB-BP750335XBE1
CSB-MP750335XBE1
CSB-EP750335XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein GTLF3B (gtlf3b) CSB-YP750336XBE
CSB-EP750336XBE
CSB-BP750336XBE
CSB-MP750336XBE
CSB-EP750336XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cell differentiation protein RCD1 homolog (rqcd1) CSB-YP750337XBE
CSB-EP750337XBE
CSB-BP750337XBE
CSB-MP750337XBE
CSB-EP750337XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis RAC-beta serine/threonine-protein kinase B (akt2-b) CSB-YP750338XBE
CSB-EP750338XBE
CSB-BP750338XBE
CSB-MP750338XBE
CSB-EP750338XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pleckstrin homology domain-containing family A member 7 (PLEKHA7), partial CSB-YP750339HU
CSB-EP750339HU
CSB-BP750339HU
CSB-MP750339HU
CSB-EP750339HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable protein BRICK1 (brk1) CSB-YP750340DIL
CSB-EP750340DIL
CSB-BP750340DIL
CSB-MP750340DIL
CSB-EP750340DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Poly (U)-binding-splicing factor PUF60-B (puf60b), partial CSB-YP750341DIL
CSB-EP750341DIL
CSB-BP750341DIL
CSB-MP750341DIL
CSB-EP750341DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable alpha-ketoglutarate-dependent dioxygenase ABH6 (alkbh6) CSB-YP750342DIL
CSB-EP750342DIL
CSB-BP750342DIL
CSB-MP750342DIL
CSB-EP750342DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Chondroitin sulfate synthase 2 (Chpf), partial CSB-YP750343MO
CSB-EP750343MO
CSB-BP750343MO
CSB-MP750343MO
CSB-EP750343MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis THAP domain-containing protein 11 (thap11) CSB-YP750344XBE
CSB-EP750344XBE
CSB-BP750344XBE
CSB-MP750344XBE
CSB-EP750344XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0536 protein C12orf66 homolog CSB-YP750345XBE
CSB-EP750345XBE
CSB-BP750345XBE
CSB-MP750345XBE
CSB-EP750345XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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