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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Picrophilus torridus Sugar fermentation stimulation protein homolog (sfsA) CSB-YP750406PAAB
CSB-EP750406PAAB
CSB-BP750406PAAB
CSB-MP750406PAAB
CSB-EP750406PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus GTP cyclohydrolase mptA (mptA) CSB-YP750407PAAB
CSB-EP750407PAAB
CSB-BP750407PAAB
CSB-MP750407PAAB
CSB-EP750407PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Phosphoenolpyruvate carboxylase (ppcA), partial CSB-YP750408PAAB
CSB-EP750408PAAB
CSB-BP750408PAAB
CSB-MP750408PAAB
CSB-EP750408PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 30S ribosomal protein S24e (rps24e) CSB-YP750409PAAB
CSB-EP750409PAAB
CSB-BP750409PAAB
CSB-MP750409PAAB
CSB-EP750409PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable cobyric acid synthase (cobQ) CSB-YP750410PAAB
CSB-EP750410PAAB
CSB-BP750410PAAB
CSB-MP750410PAAB
CSB-EP750410PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus tRNA pseudouridine synthase A (truA) CSB-YP750411PAAB
CSB-EP750411PAAB
CSB-BP750411PAAB
CSB-MP750411PAAB
CSB-EP750411PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Protoheme IX farnesyltransferase 1 (ctaB1), partial CSB-YP750412PAAB
CSB-EP750412PAAB
CSB-BP750412PAAB
CSB-MP750412PAAB
CSB-EP750412PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus DNA ligase (lig), partial CSB-YP750413PAAB
CSB-EP750413PAAB
CSB-BP750413PAAB
CSB-MP750413PAAB
CSB-EP750413PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 30S ribosomal protein S4e (rps4e) CSB-YP750414PAAB
CSB-EP750414PAAB
CSB-BP750414PAAB
CSB-MP750414PAAB
CSB-EP750414PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Protein translation factor SUI1 homolog (PTO0648) CSB-YP750415PAAB
CSB-EP750415PAAB
CSB-BP750415PAAB
CSB-MP750415PAAB
CSB-EP750415PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP750416PAAB
CSB-EP750416PAAB
CSB-BP750416PAAB
CSB-MP750416PAAB
CSB-EP750416PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 3-dehydroquinate synthase (aroB) CSB-YP750417PAAB
CSB-EP750417PAAB
CSB-BP750417PAAB
CSB-MP750417PAAB
CSB-EP750417PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus DNA polymerase II large subunit (polC), partial CSB-YP750418PAAB
CSB-EP750418PAAB
CSB-BP750418PAAB
CSB-MP750418PAAB
CSB-EP750418PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Methionine--tRNA ligase (metG), partial CSB-YP750419PAAB
CSB-EP750419PAAB
CSB-BP750419PAAB
CSB-MP750419PAAB
CSB-EP750419PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus V-type ATP synthase subunit E (atpE) CSB-YP750420PAAB
CSB-EP750420PAAB
CSB-BP750420PAAB
CSB-MP750420PAAB
CSB-EP750420PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Homoserine kinase (thrB) CSB-YP750421PAAB
CSB-EP750421PAAB
CSB-BP750421PAAB
CSB-MP750421PAAB
CSB-EP750421PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L1P (rpl1p) CSB-YP750422PAAB
CSB-EP750422PAAB
CSB-BP750422PAAB
CSB-MP750422PAAB
CSB-EP750422PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Isoleucine--tRNA ligase (ileS), partial CSB-YP750423PAAB
CSB-EP750423PAAB
CSB-BP750423PAAB
CSB-MP750423PAAB
CSB-EP750423PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L21e (rpl21e) CSB-YP750424PAAB
CSB-EP750424PAAB
CSB-BP750424PAAB
CSB-MP750424PAAB
CSB-EP750424PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus DNA-directed RNA polymerase subunit N (rpoN) CSB-YP750425PAAB
CSB-EP750425PAAB
CSB-BP750425PAAB
CSB-MP750425PAAB
CSB-EP750425PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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