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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP751811LEV
CSB-EP751811LEV
CSB-BP751811LEV
CSB-MP751811LEV
CSB-EP751811LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Probable chemoreceptor glutamine deamidase CheD 2 (cheD2) CSB-YP751812LEV
CSB-EP751812LEV
CSB-BP751812LEV
CSB-MP751812LEV
CSB-EP751812LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP751813DOT1
CSB-EP751813DOT1
CSB-BP751813DOT1
CSB-MP751813DOT1
CSB-EP751813DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin H5 (Os05g0480200, LOC_Os05g40190) CSB-YP751814OFG
CSB-EP751814OFG
CSB-BP751814OFG
CSB-MP751814OFG
CSB-EP751814OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable methylenetetrahydrofolate reductase (Os03g0815200, LOC_Os03g60090), partial CSB-YP751815OFG
CSB-EP751815OFG
CSB-BP751815OFG
CSB-MP751815OFG
CSB-EP751815OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Solanesyl-diphosphate synthase 2, chloroplastic (SPS2) CSB-YP751816OFG
CSB-EP751816OFG
CSB-BP751816OFG
CSB-MP751816OFG
CSB-EP751816OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica COBRA-like protein 2 (BC1L2), partial CSB-YP751817OFG1
CSB-EP751817OFG1
CSB-BP751817OFG1
CSB-MP751817OFG1
CSB-EP751817OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum HssA/B-like protein 12 (hssl12) CSB-YP751818DKK
CSB-EP751818DKK
CSB-BP751818DKK
CSB-MP751818DKK
CSB-EP751818DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0276139 (DDB_G0276139) CSB-YP751819DKK
CSB-EP751819DKK
CSB-BP751819DKK
CSB-MP751819DKK
CSB-EP751819DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Mitochondrial DNA repair protein recA homolog (recA) CSB-YP751820DKK
CSB-EP751820DKK
CSB-BP751820DKK
CSB-MP751820DKK
CSB-EP751820DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Frizzled/smoothened-like sans CRD protein D (fscD), partial CSB-YP751821DKK1
CSB-EP751821DKK1
CSB-BP751821DKK1
CSB-MP751821DKK1
CSB-EP751821DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum ATPase WRNIP1 (wrnip1), partial CSB-YP751822DKK
CSB-EP751822DKK
CSB-BP751822DKK
CSB-MP751822DKK
CSB-EP751822DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum COP9 signalosome complex subunit 8 (csn8) CSB-YP751823DKK
CSB-EP751823DKK
CSB-BP751823DKK
CSB-MP751823DKK
CSB-EP751823DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cysteine protease ATG4A (ATG4A) CSB-YP751824OFG
CSB-EP751824OFG
CSB-BP751824OFG
CSB-MP751824OFG
CSB-EP751824OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable ion channel CASTOR (Os03g0843600, LOC_Os03g62650), partial CSB-YP751825OFG
CSB-EP751825OFG
CSB-BP751825OFG
CSB-MP751825OFG
CSB-EP751825OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Calcineurin B-like protein 3 (CBL3) CSB-YP751826OFG
CSB-EP751826OFG
CSB-BP751826OFG
CSB-MP751826OFG
CSB-EP751826OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein transport protein Sec16B (Sec16b), partial CSB-YP751827RA
CSB-EP751827RA
CSB-BP751827RA
CSB-MP751827RA
CSB-EP751827RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein transport protein Sec16B (SEC16B), partial CSB-YP751828BO
CSB-EP751828BO
CSB-BP751828BO
CSB-MP751828BO
CSB-EP751828BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mitochondrial import receptor subunit TOM22 homolog (Tomm22), partial CSB-YP751829RA
CSB-EP751829RA
CSB-BP751829RA
CSB-MP751829RA
CSB-EP751829RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio anguillarum Na (+)-translocating NADH-quinone reductase subunit D (nqrD), partial CSB-YP751830VEV1
CSB-EP751830VEV1
CSB-BP751830VEV1
CSB-MP751830VEV1
CSB-EP751830VEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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