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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Protochlamydia amoebophila Elongation factor P 2 (efp2) CSB-YP750566PAAD
CSB-EP750566PAAD
CSB-BP750566PAAD
CSB-MP750566PAAD
CSB-EP750566PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP750567PAAD
CSB-EP750567PAAD
CSB-BP750567PAAD
CSB-MP750567PAAD
CSB-EP750567PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Acetate kinase (ackA) CSB-YP750568PAAD
CSB-EP750568PAAD
CSB-BP750568PAAD
CSB-MP750568PAAD
CSB-EP750568PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP750569PAAD
CSB-EP750569PAAD
CSB-BP750569PAAD
CSB-MP750569PAAD
CSB-EP750569PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Tyrosine--tRNA ligase (tyrS) CSB-YP750570PAAD
CSB-EP750570PAAD
CSB-BP750570PAAD
CSB-MP750570PAAD
CSB-EP750570PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Probable rRNA maturation factor (pc1054) CSB-YP750571PAAD
CSB-EP750571PAAD
CSB-BP750571PAAD
CSB-MP750571PAAD
CSB-EP750571PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP750572PAAD
CSB-EP750572PAAD
CSB-BP750572PAAD
CSB-MP750572PAAD
CSB-EP750572PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Elongation factor P 1 (efp1) CSB-YP750573PAAD
CSB-EP750573PAAD
CSB-BP750573PAAD
CSB-MP750573PAAD
CSB-EP750573PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila tRNA pseudouridine synthase A 2 (truA2) CSB-YP750574PAAD
CSB-EP750574PAAD
CSB-BP750574PAAD
CSB-MP750574PAAD
CSB-EP750574PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S16 (rpsP) CSB-YP750575PAAD
CSB-EP750575PAAD
CSB-BP750575PAAD
CSB-MP750575PAAD
CSB-EP750575PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Maf-like protein pc0610 (pc0610) CSB-YP750576PAAD
CSB-EP750576PAAD
CSB-BP750576PAAD
CSB-MP750576PAAD
CSB-EP750576PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Elongation factor Tu (tuf) CSB-YP750577PAAD
CSB-EP750577PAAD
CSB-BP750577PAAD
CSB-MP750577PAAD
CSB-EP750577PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Undecaprenyl-diphosphatase (uppP), partial CSB-YP750578PAAD1
CSB-EP750578PAAD1
CSB-BP750578PAAD1
CSB-MP750578PAAD1
CSB-EP750578PAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L15 (rplO) CSB-YP750579PAAD
CSB-EP750579PAAD
CSB-BP750579PAAD
CSB-MP750579PAAD
CSB-EP750579PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S8 (rpsH) CSB-YP750580PAAD
CSB-EP750580PAAD
CSB-BP750580PAAD
CSB-MP750580PAAD
CSB-EP750580PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L29 (rpmC) CSB-YP750581PAAD
CSB-EP750581PAAD
CSB-BP750581PAAD
CSB-MP750581PAAD
CSB-EP750581PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP750582PAAD
CSB-EP750582PAAD
CSB-BP750582PAAD
CSB-MP750582PAAD
CSB-EP750582PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Glutamyl-tRNA reductase (hemA) CSB-YP750583PAAD
CSB-EP750583PAAD
CSB-BP750583PAAD
CSB-MP750583PAAD
CSB-EP750583PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Elongation factor 4 (lepA), partial CSB-YP750584PAAD
CSB-EP750584PAAD
CSB-BP750584PAAD
CSB-MP750584PAAD
CSB-EP750584PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP750585PAAD
CSB-EP750585PAAD
CSB-BP750585PAAD
CSB-MP750585PAAD
CSB-EP750585PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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