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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photobacterium profundum Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP750506PIG
CSB-EP750506PIG
CSB-BP750506PIG
CSB-MP750506PIG
CSB-EP750506PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Transcriptional repressor NrdR (nrdR) CSB-YP750507PIG
CSB-EP750507PIG
CSB-BP750507PIG
CSB-MP750507PIG
CSB-EP750507PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP750508PIG
CSB-EP750508PIG
CSB-BP750508PIG
CSB-MP750508PIG
CSB-EP750508PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum HTH-type transcriptional regulator iscR (iscR) CSB-YP750509PIG
CSB-EP750509PIG
CSB-BP750509PIG
CSB-MP750509PIG
CSB-EP750509PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Purine nucleoside phosphorylase DeoD-type 1 (deoD1) CSB-YP750510PIG
CSB-EP750510PIG
CSB-BP750510PIG
CSB-MP750510PIG
CSB-EP750510PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Translation initiation factor IF-2 (infB), partial CSB-YP750511PIG
CSB-EP750511PIG
CSB-BP750511PIG
CSB-MP750511PIG
CSB-EP750511PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP750512PIG
CSB-EP750512PIG
CSB-BP750512PIG
CSB-MP750512PIG
CSB-EP750512PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Probable endonuclease 4 (nfo) CSB-YP750513PIG
CSB-EP750513PIG
CSB-BP750513PIG
CSB-MP750513PIG
CSB-EP750513PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP750514PIG
CSB-EP750514PIG
CSB-BP750514PIG
CSB-MP750514PIG
CSB-EP750514PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Probable cytosol aminopeptidase (pepA), partial CSB-YP750515PIG
CSB-EP750515PIG
CSB-BP750515PIG
CSB-MP750515PIG
CSB-EP750515PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Xylose import ATP-binding protein XylG (xylG), partial CSB-YP750516PIG
CSB-EP750516PIG
CSB-BP750516PIG
CSB-MP750516PIG
CSB-EP750516PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP750517PIG
CSB-EP750517PIG
CSB-BP750517PIG
CSB-MP750517PIG
CSB-EP750517PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Thiamine import ATP-binding protein ThiQ (thiQ) CSB-YP750518PIG
CSB-EP750518PIG
CSB-BP750518PIG
CSB-MP750518PIG
CSB-EP750518PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Arginine repressor (argR) CSB-YP750519PIG
CSB-EP750519PIG
CSB-BP750519PIG
CSB-MP750519PIG
CSB-EP750519PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L36 (rpmJ) CSB-YP750520PIG
CSB-EP750520PIG
CSB-BP750520PIG
CSB-MP750520PIG
CSB-EP750520PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L23 (rplW) CSB-YP750521PIG
CSB-EP750521PIG
CSB-BP750521PIG
CSB-MP750521PIG
CSB-EP750521PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-dehydroquinate synthase (aroB) CSB-YP750522PIG
CSB-EP750522PIG
CSB-BP750522PIG
CSB-MP750522PIG
CSB-EP750522PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein-export protein SecB (secB) CSB-YP750523PIG
CSB-EP750523PIG
CSB-BP750523PIG
CSB-MP750523PIG
CSB-EP750523PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP750524PIG
CSB-EP750524PIG
CSB-BP750524PIG
CSB-MP750524PIG
CSB-EP750524PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Thiosulfate sulfurtransferase glpE (glpE) CSB-YP750525PIG
CSB-EP750525PIG
CSB-BP750525PIG
CSB-MP750525PIG
CSB-EP750525PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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