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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma mycoides subsp. mycoides SC ATP synthase subunit beta (atpD) CSB-YP750626MLS
CSB-EP750626MLS
CSB-BP750626MLS
CSB-MP750626MLS
CSB-EP750626MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L23 (rplW) CSB-YP750627MLS
CSB-EP750627MLS
CSB-BP750627MLS
CSB-MP750627MLS
CSB-EP750627MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L13 (rplM) CSB-YP750628MLS
CSB-EP750628MLS
CSB-BP750628MLS
CSB-MP750628MLS
CSB-EP750628MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Putative agmatine deiminase (aguA) CSB-YP750629MLS
CSB-EP750629MLS
CSB-BP750629MLS
CSB-MP750629MLS
CSB-EP750629MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Arginine--tRNA ligase (argS), partial CSB-YP750630MLS
CSB-EP750630MLS
CSB-BP750630MLS
CSB-MP750630MLS
CSB-EP750630MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 50S ribosomal protein L21 (rplU) CSB-YP750631MLS
CSB-EP750631MLS
CSB-BP750631MLS
CSB-MP750631MLS
CSB-EP750631MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Signal recognition particle receptor FtsY (ftsY) CSB-YP750632MLS
CSB-EP750632MLS
CSB-BP750632MLS
CSB-MP750632MLS
CSB-EP750632MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Ribonuclease 3 (rnc) CSB-YP750633MLS
CSB-EP750633MLS
CSB-BP750633MLS
CSB-MP750633MLS
CSB-EP750633MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Lon protease (lon), partial CSB-YP750634MLS
CSB-EP750634MLS
CSB-BP750634MLS
CSB-MP750634MLS
CSB-EP750634MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP750635MLS
CSB-EP750635MLS
CSB-BP750635MLS
CSB-MP750635MLS
CSB-EP750635MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Ribosome maturation factor RimP (rimP) CSB-YP750636MLS
CSB-EP750636MLS
CSB-BP750636MLS
CSB-MP750636MLS
CSB-EP750636MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Probable GTP-binding protein EngB (engB) CSB-YP750637MLS
CSB-EP750637MLS
CSB-BP750637MLS
CSB-MP750637MLS
CSB-EP750637MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP750638MLS
CSB-EP750638MLS
CSB-BP750638MLS
CSB-MP750638MLS
CSB-EP750638MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC 30S ribosomal protein S20 (rpsT) CSB-YP750639MLS
CSB-EP750639MLS
CSB-BP750639MLS
CSB-MP750639MLS
CSB-EP750639MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Ribonuclease M5 (rnmV) CSB-YP750640MLS
CSB-EP750640MLS
CSB-BP750640MLS
CSB-MP750640MLS
CSB-EP750640MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Putative uncharacterized protein B8J22.070 (B8J22.070, NCU09235, NCU10996) CSB-YP750641NHA
CSB-EP750641NHA
CSB-BP750641NHA
CSB-MP750641NHA
CSB-EP750641NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Formin-like protein 18 (FH18), partial CSB-YP750642OFG
CSB-EP750642OFG
CSB-BP750642OFG
CSB-MP750642OFG
CSB-EP750642OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable toxin MazF1 (mazF1) CSB-YP750643MVZ
CSB-EP750643MVZ
CSB-BP750643MVZ
CSB-MP750643MVZ
CSB-EP750643MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C4orf40 (C4orf40) CSB-YP750644HU
CSB-EP750644HU
CSB-BP750644HU
CSB-MP750644HU
CSB-EP750644HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Proline-rich protein 5-like (PRR5L) CSB-YP750645HU
CSB-EP750645HU
CSB-BP750645HU
CSB-MP750645HU
CSB-EP750645HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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