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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Urease subunit gamma (ureA) CSB-YP751471BQN
CSB-EP751471BQN
CSB-BP751471BQN
CSB-MP751471BQN
CSB-EP751471BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein P (sspP) CSB-YP751472BQN
CSB-EP751472BQN
CSB-BP751472BQN
CSB-MP751472BQN
CSB-EP751472BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0061 protein BCE_3522 (BCE_3522) CSB-YP751473BQN
CSB-EP751473BQN
CSB-BP751473BQN
CSB-MP751473BQN
CSB-EP751473BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein ArsC 2 (arsC2) CSB-YP751474BQN
CSB-EP751474BQN
CSB-BP751474BQN
CSB-MP751474BQN
CSB-EP751474BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP751475BQN
CSB-EP751475BQN
CSB-BP751475BQN
CSB-MP751475BQN
CSB-EP751475BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol-3-phosphate acyltransferase 1 (plsY1), partial CSB-YP751476BQN1
CSB-EP751476BQN1
CSB-BP751476BQN1
CSB-MP751476BQN1
CSB-EP751476BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP751477BQN
CSB-EP751477BQN
CSB-BP751477BQN
CSB-MP751477BQN
CSB-EP751477BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP751478BQN
CSB-EP751478BQN
CSB-BP751478BQN
CSB-MP751478BQN
CSB-EP751478BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP751479BQN
CSB-EP751479BQN
CSB-BP751479BQN
CSB-MP751479BQN
CSB-EP751479BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell cycle protein GpsB (gpsB) CSB-YP751480BQN
CSB-EP751480BQN
CSB-BP751480BQN
CSB-MP751480BQN
CSB-EP751480BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction resolvase recU (recU) CSB-YP751481BQN
CSB-EP751481BQN
CSB-BP751481BQN
CSB-MP751481BQN
CSB-EP751481BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0302 protein BCE_1647 (BCE_1647) CSB-YP751482BQN
CSB-EP751482BQN
CSB-BP751482BQN
CSB-MP751482BQN
CSB-EP751482BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP751483BQN
CSB-EP751483BQN
CSB-BP751483BQN
CSB-MP751483BQN
CSB-EP751483BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP751484BQN
CSB-EP751484BQN
CSB-BP751484BQN
CSB-MP751484BQN
CSB-EP751484BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0180 protein BCE_1513 (BCE_1513) CSB-YP751485BQN
CSB-EP751485BQN
CSB-BP751485BQN
CSB-MP751485BQN
CSB-EP751485BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP751486BQN
CSB-EP751486BQN
CSB-BP751486BQN
CSB-MP751486BQN
CSB-EP751486BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP751487BQN
CSB-EP751487BQN
CSB-BP751487BQN
CSB-MP751487BQN
CSB-EP751487BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP751488BQN
CSB-EP751488BQN
CSB-BP751488BQN
CSB-MP751488BQN
CSB-EP751488BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0344 protein BCE_1257 (BCE_1257), partial CSB-YP751489BQN1
CSB-EP751489BQN1
CSB-BP751489BQN1
CSB-MP751489BQN1
CSB-EP751489BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus HTH-type transcriptional regulator hpr (hpr) CSB-YP751490BQN
CSB-EP751490BQN
CSB-BP751490BQN
CSB-MP751490BQN
CSB-EP751490BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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