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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP752474CKA
CSB-EP752474CKA
CSB-BP752474CKA
CSB-MP752474CKA
CSB-EP752474CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glucose-6-phosphate isomerase 2 (pgi2), partial CSB-YP752475CKA
CSB-EP752475CKA
CSB-BP752475CKA
CSB-MP752475CKA
CSB-EP752475CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phosphoserine aminotransferase (serC) CSB-YP752476CKA
CSB-EP752476CKA
CSB-BP752476CKA
CSB-MP752476CKA
CSB-EP752476CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Uridylate kinase (pyrH) CSB-YP752477CKA
CSB-EP752477CKA
CSB-BP752477CKA
CSB-MP752477CKA
CSB-EP752477CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 3-isopropylmalate dehydratase small subunit 1 (leuD1) CSB-YP752478CKA
CSB-EP752478CKA
CSB-BP752478CKA
CSB-MP752478CKA
CSB-EP752478CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Elongation factor 4 (lepA), partial CSB-YP752479CKA
CSB-EP752479CKA
CSB-BP752479CKA
CSB-MP752479CKA
CSB-EP752479CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP752480CKA
CSB-EP752480CKA
CSB-BP752480CKA
CSB-MP752480CKA
CSB-EP752480CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Translation initiation factor IF-1 1 (infA1) CSB-YP752481CKA
CSB-EP752481CKA
CSB-BP752481CKA
CSB-MP752481CKA
CSB-EP752481CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP752482CKA
CSB-EP752482CKA
CSB-BP752482CKA
CSB-MP752482CKA
CSB-EP752482CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Flagellar P-ring protein 1 (flgI1) CSB-YP752483CKA
CSB-EP752483CKA
CSB-BP752483CKA
CSB-MP752483CKA
CSB-EP752483CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Protein FdhD homolog (fdhD) CSB-YP752484CKA
CSB-EP752484CKA
CSB-BP752484CKA
CSB-MP752484CKA
CSB-EP752484CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Alanine--tRNA ligase (alaS), partial CSB-YP752485CKA
CSB-EP752485CKA
CSB-BP752485CKA
CSB-MP752485CKA
CSB-EP752485CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Acetate kinase (ackA) CSB-YP752486CKA
CSB-EP752486CKA
CSB-BP752486CKA
CSB-MP752486CKA
CSB-EP752486CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP752487CKA
CSB-EP752487CKA
CSB-BP752487CKA
CSB-MP752487CKA
CSB-EP752487CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP752488CKA
CSB-EP752488CKA
CSB-BP752488CKA
CSB-MP752488CKA
CSB-EP752488CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UvrABC system protein C (uvrC), partial CSB-YP752489CKA
CSB-EP752489CKA
CSB-BP752489CKA
CSB-MP752489CKA
CSB-EP752489CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP752490CKA
CSB-EP752490CKA
CSB-BP752490CKA
CSB-MP752490CKA
CSB-EP752490CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP752491CKA
CSB-EP752491CKA
CSB-BP752491CKA
CSB-MP752491CKA
CSB-EP752491CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Chaperone protein hscA homolog (hscA), partial CSB-YP752492CKA
CSB-EP752492CKA
CSB-BP752492CKA
CSB-MP752492CKA
CSB-EP752492CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon (cheB2) CSB-YP752493CKA
CSB-EP752493CKA
CSB-BP752493CKA
CSB-MP752493CKA
CSB-EP752493CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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