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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Dihydroxy-acid dehydratase (ilvD), partial CSB-YP751431LEV
CSB-EP751431LEV
CSB-BP751431LEV
CSB-MP751431LEV
CSB-EP751431LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Potassium-transporting ATPase B chain (kdpB), partial CSB-YP751432LEV1
CSB-EP751432LEV1
CSB-BP751432LEV1
CSB-MP751432LEV1
CSB-EP751432LEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Tryptophan synthase alpha chain (trpA) CSB-YP751433LEV
CSB-EP751433LEV
CSB-BP751433LEV
CSB-MP751433LEV
CSB-EP751433LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Proline--tRNA ligase (proS), partial CSB-YP751434LEV
CSB-EP751434LEV
CSB-BP751434LEV
CSB-MP751434LEV
CSB-EP751434LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Ribosome-recycling factor (frr) CSB-YP751435LEV
CSB-EP751435LEV
CSB-BP751435LEV
CSB-MP751435LEV
CSB-EP751435LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 30S ribosomal protein S9 (rpsI) CSB-YP751436LEV
CSB-EP751436LEV
CSB-BP751436LEV
CSB-MP751436LEV
CSB-EP751436LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L1 (rplA) CSB-YP751437LEV
CSB-EP751437LEV
CSB-BP751437LEV
CSB-MP751437LEV
CSB-EP751437LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Lon protease (lon), partial CSB-YP751438LEV
CSB-EP751438LEV
CSB-BP751438LEV
CSB-MP751438LEV
CSB-EP751438LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 30S ribosomal protein S20 (rpsT) CSB-YP751439LEV
CSB-EP751439LEV
CSB-BP751439LEV
CSB-MP751439LEV
CSB-EP751439LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Elongation factor G (fusA), partial CSB-YP751440LEV
CSB-EP751440LEV
CSB-BP751440LEV
CSB-MP751440LEV
CSB-EP751440LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Chorismate synthase (aroC) CSB-YP751441LEV
CSB-EP751441LEV
CSB-BP751441LEV
CSB-MP751441LEV
CSB-EP751441LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP751442LEV
CSB-EP751442LEV
CSB-BP751442LEV
CSB-MP751442LEV
CSB-EP751442LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Chromosomal replication initiator protein DnaA (dnaA) CSB-YP751443LEV
CSB-EP751443LEV
CSB-BP751443LEV
CSB-MP751443LEV
CSB-EP751443LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA modification GTPase MnmE (mnmE) CSB-YP751444BQN
CSB-EP751444BQN
CSB-BP751444BQN
CSB-MP751444BQN
CSB-EP751444BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Homoserine O-succinyltransferase (metA) CSB-YP751445BQN
CSB-EP751445BQN
CSB-BP751445BQN
CSB-MP751445BQN
CSB-EP751445BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP751446BQN
CSB-EP751446BQN
CSB-BP751446BQN
CSB-MP751446BQN
CSB-EP751446BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative sporulation transcription regulator WhiA (whiA) CSB-YP751447BQN
CSB-EP751447BQN
CSB-BP751447BQN
CSB-MP751447BQN
CSB-EP751447BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylenolpyruvoylglucosamine reductase 2 (murB2) CSB-YP751448BQN
CSB-EP751448BQN
CSB-BP751448BQN
CSB-MP751448BQN
CSB-EP751448BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP751449BQN
CSB-EP751449BQN
CSB-BP751449BQN
CSB-MP751449BQN
CSB-EP751449BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-ribosylhomocysteine lyase (luxS) CSB-YP751450BQN
CSB-EP751450BQN
CSB-BP751450BQN
CSB-MP751450BQN
CSB-EP751450BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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