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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermus thermophilus 50S ribosomal protein L25 (rplY) CSB-YP751391TNS
CSB-EP751391TNS
CSB-BP751391TNS
CSB-MP751391TNS
CSB-EP751391TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP751392TNS
CSB-EP751392TNS
CSB-BP751392TNS
CSB-MP751392TNS
CSB-EP751392TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Chaperone protein ClpB (clpB), partial CSB-YP751393TNS
CSB-EP751393TNS
CSB-BP751393TNS
CSB-MP751393TNS
CSB-EP751393TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Endonuclease V (nfi) CSB-YP751394TNS
CSB-EP751394TNS
CSB-BP751394TNS
CSB-MP751394TNS
CSB-EP751394TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Bifunctional protein FolD (folD) CSB-YP751395TNS
CSB-EP751395TNS
CSB-BP751395TNS
CSB-MP751395TNS
CSB-EP751395TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP751396TNS
CSB-EP751396TNS
CSB-BP751396TNS
CSB-MP751396TNS
CSB-EP751396TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Lysine--tRNA ligase (lysS) CSB-YP751397TNS
CSB-EP751397TNS
CSB-BP751397TNS
CSB-MP751397TNS
CSB-EP751397TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP751398TNS
CSB-EP751398TNS
CSB-BP751398TNS
CSB-MP751398TNS
CSB-EP751398TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Dephospho-CoA kinase (coaE) CSB-YP751399TNS
CSB-EP751399TNS
CSB-BP751399TNS
CSB-MP751399TNS
CSB-EP751399TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Asparagine--tRNA ligase (asnS) CSB-YP751400TNS
CSB-EP751400TNS
CSB-BP751400TNS
CSB-MP751400TNS
CSB-EP751400TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Aspartate--tRNA ligase (aspS), partial CSB-YP751401TNS
CSB-EP751401TNS
CSB-BP751401TNS
CSB-MP751401TNS
CSB-EP751401TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP751402TNS
CSB-EP751402TNS
CSB-BP751402TNS
CSB-MP751402TNS
CSB-EP751402TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Uridine kinase (udk) CSB-YP751403TNS
CSB-EP751403TNS
CSB-BP751403TNS
CSB-MP751403TNS
CSB-EP751403TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Glycine--tRNA ligase (glyQS), partial CSB-YP751404TNS
CSB-EP751404TNS
CSB-BP751404TNS
CSB-MP751404TNS
CSB-EP751404TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 2-aminoadipate transaminase (lysN) CSB-YP751405TNS
CSB-EP751405TNS
CSB-BP751405TNS
CSB-MP751405TNS
CSB-EP751405TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni S-methyl-5'-thioadenosine phosphorylase (mtnP) CSB-YP751406LEV
CSB-EP751406LEV
CSB-BP751406LEV
CSB-MP751406LEV
CSB-EP751406LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glutamate--tRNA ligase (gltX), partial CSB-YP751407LEV
CSB-EP751407LEV
CSB-BP751407LEV
CSB-MP751407LEV
CSB-EP751407LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Flagellar assembly factor FliW (fliW) CSB-YP751408LEV
CSB-EP751408LEV
CSB-BP751408LEV
CSB-MP751408LEV
CSB-EP751408LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glucose-6-phosphate isomerase (pgi) CSB-YP751409LEV
CSB-EP751409LEV
CSB-BP751409LEV
CSB-MP751409LEV
CSB-EP751409LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Shikimate dehydrogenase (aroE) CSB-YP751410LEV
CSB-EP751410LEV
CSB-BP751410LEV
CSB-MP751410LEV
CSB-EP751410LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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