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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii GPI-anchored wall transfer protein 1 (GWT1), partial CSB-YP751731DOT
CSB-EP751731DOT
CSB-BP751731DOT
CSB-MP751731DOT
CSB-EP751731DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii FK506-binding protein 1 (FPR1) CSB-YP751732DOT
CSB-EP751732DOT
CSB-BP751732DOT
CSB-MP751732DOT
CSB-EP751732DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Lon protease homolog, mitochondrial (PIM1), partial CSB-YP751733DOT
CSB-EP751733DOT
CSB-BP751733DOT
CSB-MP751733DOT
CSB-EP751733DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribosome biogenesis protein NSA1 (NSA1) CSB-YP751734DOT
CSB-EP751734DOT
CSB-BP751734DOT
CSB-MP751734DOT
CSB-EP751734DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Spindle assembly checkpoint component MAD1 (MAD1), partial CSB-YP751735DOT
CSB-EP751735DOT
CSB-BP751735DOT
CSB-MP751735DOT
CSB-EP751735DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Monopolar spindle protein 2 (MPS2), partial CSB-YP751736DOT1
CSB-EP751736DOT1
CSB-BP751736DOT1
CSB-MP751736DOT1
CSB-EP751736DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii U2 small nuclear ribonucleoprotein A' (LEA1) CSB-YP751737DOT
CSB-EP751737DOT
CSB-BP751737DOT
CSB-MP751737DOT
CSB-EP751737DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii UDP-galactose transporter homolog 1 (HUT1), partial CSB-YP751738DOT
CSB-EP751738DOT
CSB-BP751738DOT
CSB-MP751738DOT
CSB-EP751738DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Survival factor 1 (SVF1) CSB-YP751739DOT
CSB-EP751739DOT
CSB-BP751739DOT
CSB-MP751739DOT
CSB-EP751739DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 9 (CSE2) CSB-YP751740DOT
CSB-EP751740DOT
CSB-BP751740DOT
CSB-MP751740DOT
CSB-EP751740DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone H2A.Z-specific chaperone CHZ1 (CHZ1) CSB-YP751741DOT
CSB-EP751741DOT
CSB-BP751741DOT
CSB-MP751741DOT
CSB-EP751741DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Altered inheritance of mitochondria protein 37, mitochondrial (AIM37) CSB-YP751742DOT
CSB-EP751742DOT
CSB-BP751742DOT
CSB-MP751742DOT
CSB-EP751742DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein EFR3 (EFR3), partial CSB-YP751743DOT
CSB-EP751743DOT
CSB-BP751743DOT
CSB-MP751743DOT
CSB-EP751743DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-rRNA-processing protein ESF1 (ESF1), partial CSB-YP751744DOT
CSB-EP751744DOT
CSB-BP751744DOT
CSB-MP751744DOT
CSB-EP751744DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytochrome c lysine N-methyltransferase 1 (CTM1) CSB-YP751745DOT
CSB-EP751745DOT
CSB-BP751745DOT
CSB-MP751745DOT
CSB-EP751745DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Heterogeneous nuclear rnp K-like protein 2 (HEK2) CSB-YP751746DOT
CSB-EP751746DOT
CSB-BP751746DOT
CSB-MP751746DOT
CSB-EP751746DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Lysophospholipase NTE1 (NTE1), partial CSB-YP751747DOT
CSB-EP751747DOT
CSB-BP751747DOT
CSB-MP751747DOT
CSB-EP751747DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Calcineurin subunit B (CNB1) CSB-YP751748DOT
CSB-EP751748DOT
CSB-BP751748DOT
CSB-MP751748DOT
CSB-EP751748DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial ATPase complex subunit ATP10 (ATP10) CSB-YP751749DOT
CSB-EP751749DOT
CSB-BP751749DOT
CSB-MP751749DOT
CSB-EP751749DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 5-amino-6- (5-phosphoribosylamino)uracil reductase (RIB7) CSB-YP751750DOT
CSB-EP751750DOT
CSB-BP751750DOT
CSB-MP751750DOT
CSB-EP751750DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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