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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii Telomere replication protein EST3 (EST3) CSB-YP751771DOT
CSB-EP751771DOT
CSB-BP751771DOT
CSB-MP751771DOT
CSB-EP751771DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial aspartate-glutamate transporter AGC1 (AGC1), partial CSB-YP751772DOT
CSB-EP751772DOT
CSB-BP751772DOT
CSB-MP751772DOT
CSB-EP751772DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii cAMP-dependent protein kinase regulatory subunit (PKAR) CSB-YP751773DOT
CSB-EP751773DOT
CSB-BP751773DOT
CSB-MP751773DOT
CSB-EP751773DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor CWC25 (CWC25) CSB-YP751774DOT
CSB-EP751774DOT
CSB-BP751774DOT
CSB-MP751774DOT
CSB-EP751774DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Acetylornithine aminotransferase, mitochondrial (ARG8), partial CSB-YP751775DOT
CSB-EP751775DOT
CSB-BP751775DOT
CSB-MP751775DOT
CSB-EP751775DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Conserved oligomeric Golgi complex subunit 6 (COG6), partial CSB-YP751776DOT
CSB-EP751776DOT
CSB-BP751776DOT
CSB-MP751776DOT
CSB-EP751776DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Metacaspase-1 (MCA1), partial CSB-YP751777DOT
CSB-EP751777DOT
CSB-BP751777DOT
CSB-MP751777DOT
CSB-EP751777DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein STU1 (STU1), partial CSB-YP751778DOT
CSB-EP751778DOT
CSB-BP751778DOT
CSB-MP751778DOT
CSB-EP751778DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RNA polymerase II transcription factor B subunit 4 (TFB4) CSB-YP751779DOT
CSB-EP751779DOT
CSB-BP751779DOT
CSB-MP751779DOT
CSB-EP751779DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Spliceosomal protein DIB1 (DIB1) CSB-YP751780DOT
CSB-EP751780DOT
CSB-BP751780DOT
CSB-MP751780DOT
CSB-EP751780DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Glucose-repressible alcohol dehydrogenase transcriptional effector (CCR4), partial CSB-YP751781DOT
CSB-EP751781DOT
CSB-BP751781DOT
CSB-MP751781DOT
CSB-EP751781DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Phosphatidylinositol transfer protein SFH5 (SFH5) CSB-YP751782DOT
CSB-EP751782DOT
CSB-BP751782DOT
CSB-MP751782DOT
CSB-EP751782DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Chromatin modification-related protein EAF6 (EAF6) CSB-YP751783DOT
CSB-EP751783DOT
CSB-BP751783DOT
CSB-MP751783DOT
CSB-EP751783DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribonuclease T2-like (RNY1) CSB-YP751784DOT
CSB-EP751784DOT
CSB-BP751784DOT
CSB-MP751784DOT
CSB-EP751784DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GrpE protein homolog, mitochondrial (mge1), partial CSB-YP751785DOT
CSB-EP751785DOT
CSB-BP751785DOT
CSB-MP751785DOT
CSB-EP751785DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DBP5 (DBP5) CSB-YP751786DOT
CSB-EP751786DOT
CSB-BP751786DOT
CSB-MP751786DOT
CSB-EP751786DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Dimethyladenosine transferase (DIM1) CSB-YP751787DOT
CSB-EP751787DOT
CSB-BP751787DOT
CSB-MP751787DOT
CSB-EP751787DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Transcription initiation factor TFIID subunit 4 (TAF4) CSB-YP751788DOT
CSB-EP751788DOT
CSB-BP751788DOT
CSB-MP751788DOT
CSB-EP751788DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribosome biogenesis protein NSA2 (NSA2) CSB-YP751789DOT
CSB-EP751789DOT
CSB-BP751789DOT
CSB-MP751789DOT
CSB-EP751789DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii PX domain-containing protein YPT35 (YPT35) CSB-YP751790DOT
CSB-EP751790DOT
CSB-BP751790DOT
CSB-MP751790DOT
CSB-EP751790DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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