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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Helicobacter hepaticus Peptide chain release factor 1 (prfA) CSB-YP752936HCO
CSB-EP752936HCO
CSB-BP752936HCO
CSB-MP752936HCO
CSB-EP752936HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Porphobilinogen deaminase (hemC) CSB-YP752937HCO
CSB-EP752937HCO
CSB-BP752937HCO
CSB-MP752937HCO
CSB-EP752937HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Magnesium transport protein CorA (corA), partial CSB-YP752938HCO1
CSB-EP752938HCO1
CSB-BP752938HCO1
CSB-MP752938HCO1
CSB-EP752938HCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Probable transcriptional regulatory protein HH_1604 (HH_1604) CSB-YP752939HCO
CSB-EP752939HCO
CSB-BP752939HCO
CSB-MP752939HCO
CSB-EP752939HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Bifunctional enzyme IspD/IspF (ispDF) CSB-YP752940HCO
CSB-EP752940HCO
CSB-BP752940HCO
CSB-MP752940HCO
CSB-EP752940HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Phosphoheptose isomerase (gmhA) CSB-YP752941HCO
CSB-EP752941HCO
CSB-BP752941HCO
CSB-MP752941HCO
CSB-EP752941HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP752942HCO
CSB-EP752942HCO
CSB-BP752942HCO
CSB-MP752942HCO
CSB-EP752942HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 30S ribosomal protein S13 (rpsM) CSB-YP752943HCO
CSB-EP752943HCO
CSB-BP752943HCO
CSB-MP752943HCO
CSB-EP752943HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 30S ribosomal protein S17 (rpsQ) CSB-YP752944HCO
CSB-EP752944HCO
CSB-BP752944HCO
CSB-MP752944HCO
CSB-EP752944HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 50S ribosomal protein L23 (rplW) CSB-YP752945HCO
CSB-EP752945HCO
CSB-BP752945HCO
CSB-MP752945HCO
CSB-EP752945HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Phosphopantetheine adenylyltransferase (coaD) CSB-YP752946HCO
CSB-EP752946HCO
CSB-BP752946HCO
CSB-MP752946HCO
CSB-EP752946HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Acetate kinase (ackA) CSB-YP752947HCO
CSB-EP752947HCO
CSB-BP752947HCO
CSB-MP752947HCO
CSB-EP752947HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP752948HCO
CSB-EP752948HCO
CSB-BP752948HCO
CSB-MP752948HCO
CSB-EP752948HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP752949HCO
CSB-EP752949HCO
CSB-BP752949HCO
CSB-MP752949HCO
CSB-EP752949HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus UPF0324 membrane protein HH_1161 (HH_1161), partial CSB-YP752950HCO
CSB-EP752950HCO
CSB-BP752950HCO
CSB-MP752950HCO
CSB-EP752950HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP752951HCO
CSB-EP752951HCO
CSB-BP752951HCO
CSB-MP752951HCO
CSB-EP752951HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP752952HCO
CSB-EP752952HCO
CSB-BP752952HCO
CSB-MP752952HCO
CSB-EP752952HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP752953HCO1
CSB-EP752953HCO1
CSB-BP752953HCO1
CSB-MP752953HCO1
CSB-EP752953HCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Queuine tRNA-ribosyltransferase (tgt) CSB-YP752954HCO
CSB-EP752954HCO
CSB-BP752954HCO
CSB-MP752954HCO
CSB-EP752954HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Histidinol-phosphate aminotransferase (hisC) CSB-YP752955HCO
CSB-EP752955HCO
CSB-BP752955HCO
CSB-MP752955HCO
CSB-EP752955HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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