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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Picrophilus torridus Serine--tRNA ligase (serS) CSB-YP753777PAAB
CSB-EP753777PAAB
CSB-BP753777PAAB
CSB-MP753777PAAB
CSB-EP753777PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Aspartate--tRNA ligase (aspS) CSB-YP753778PAAB
CSB-EP753778PAAB
CSB-BP753778PAAB
CSB-MP753778PAAB
CSB-EP753778PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable thymidylate kinase (tmk) CSB-YP753779PAAB
CSB-EP753779PAAB
CSB-BP753779PAAB
CSB-MP753779PAAB
CSB-EP753779PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 30S ribosomal protein S8e (rps8e) CSB-YP753780PAAB
CSB-EP753780PAAB
CSB-BP753780PAAB
CSB-MP753780PAAB
CSB-EP753780PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP753781PAAB
CSB-EP753781PAAB
CSB-BP753781PAAB
CSB-MP753781PAAB
CSB-EP753781PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Replication factor C large subunit (rfcL) CSB-YP753782PAAB
CSB-EP753782PAAB
CSB-BP753782PAAB
CSB-MP753782PAAB
CSB-EP753782PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP753783PAAB
CSB-EP753783PAAB
CSB-BP753783PAAB
CSB-MP753783PAAB
CSB-EP753783PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L10e (rpl10e) CSB-YP753784PAAB
CSB-EP753784PAAB
CSB-BP753784PAAB
CSB-MP753784PAAB
CSB-EP753784PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable tRNA pseudouridine synthase B (truB) CSB-YP753785PAAB
CSB-EP753785PAAB
CSB-BP753785PAAB
CSB-MP753785PAAB
CSB-EP753785PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L6P (rpl6p) CSB-YP753786PAAB
CSB-EP753786PAAB
CSB-BP753786PAAB
CSB-MP753786PAAB
CSB-EP753786PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L14P (rpl14p) CSB-YP753787PAAB
CSB-EP753787PAAB
CSB-BP753787PAAB
CSB-MP753787PAAB
CSB-EP753787PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L22P (rpl22p) CSB-YP753788PAAB
CSB-EP753788PAAB
CSB-BP753788PAAB
CSB-MP753788PAAB
CSB-EP753788PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 50S ribosomal protein L3P (rpl3p) CSB-YP753789PAAB
CSB-EP753789PAAB
CSB-BP753789PAAB
CSB-MP753789PAAB
CSB-EP753789PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus UPF0095 protein PTO0617 (PTO0617) CSB-YP753790PAAB
CSB-EP753790PAAB
CSB-BP753790PAAB
CSB-MP753790PAAB
CSB-EP753790PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Ribose-5-phosphate isomerase A (rpiA) CSB-YP753791PAAB
CSB-EP753791PAAB
CSB-BP753791PAAB
CSB-MP753791PAAB
CSB-EP753791PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Probable octanoyltransferase 1 (lipB1) CSB-YP753792PAAB
CSB-EP753792PAAB
CSB-BP753792PAAB
CSB-MP753792PAAB
CSB-EP753792PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus TATA-box-binding protein (tbp) CSB-YP753793PAAB
CSB-EP753793PAAB
CSB-BP753793PAAB
CSB-MP753793PAAB
CSB-EP753793PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Lysine--tRNA ligase (lysS), partial CSB-YP753794PAAB
CSB-EP753794PAAB
CSB-BP753794PAAB
CSB-MP753794PAAB
CSB-EP753794PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Elongation factor 1-alpha (tuf) CSB-YP753795PAAB
CSB-EP753795PAAB
CSB-BP753795PAAB
CSB-MP753795PAAB
CSB-EP753795PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus UPF0219 protein PTO0384 (PTO0384) CSB-YP753796PAAB
CSB-EP753796PAAB
CSB-BP753796PAAB
CSB-MP753796PAAB
CSB-EP753796PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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