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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Fumarylacetoacetate hydrolase domain-containing protein 2B (FAHD2B) CSB-YP754177HU
CSB-EP754177HU
CSB-BP754177HU
CSB-MP754177HU
CSB-EP754177HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Calcium/calmodulin-dependent protein kinase type 1B (PNCK) CSB-YP754178HU
CSB-EP754178HU
CSB-BP754178HU
CSB-MP754178HU
CSB-EP754178HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Forkhead box protein I1 (foxi1) CSB-YP754179XBF
CSB-EP754179XBF
CSB-BP754179XBF
CSB-MP754179XBF
CSB-EP754179XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis V-type proton ATPase subunit d 2 (atp6v0d2) CSB-YP754180XBF
CSB-EP754180XBF
CSB-BP754180XBF
CSB-MP754180XBF
CSB-EP754180XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C16orf46 (C16orf46) CSB-YP754181HU
CSB-EP754181HU
CSB-BP754181HU
CSB-MP754181HU
CSB-EP754181HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative ribosome-binding factor A, mitochondrial (Rbfa) CSB-YP754182MO
CSB-EP754182MO
CSB-BP754182MO
CSB-MP754182MO
CSB-EP754182MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Casein kinase I isoform delta-B (csnk1db) CSB-YP754183DIL
CSB-EP754183DIL
CSB-BP754183DIL
CSB-MP754183DIL
CSB-EP754183DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Kinesin-like protein KIF22 (kif22), partial CSB-YP754184XBF
CSB-EP754184XBF
CSB-BP754184XBF
CSB-MP754184XBF
CSB-EP754184XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DnaJ homolog subfamily C member 24 (DNAJC24) CSB-YP754185HU
CSB-EP754185HU
CSB-BP754185HU
CSB-MP754185HU
CSB-EP754185HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis MAP kinase-interacting serine/threonine-protein kinase 2 (mknk2) CSB-YP754186XBE
CSB-EP754186XBE
CSB-BP754186XBE
CSB-MP754186XBE
CSB-EP754186XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NIPA-like protein 3 (NIPAL3), partial CSB-YP754187HU
CSB-EP754187HU
CSB-BP754187HU
CSB-MP754187HU
CSB-EP754187HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Pre-mRNA-splicing regulator WTAP (wtap) CSB-YP754188XBF
CSB-EP754188XBF
CSB-BP754188XBF
CSB-MP754188XBF
CSB-EP754188XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio LMBR1 domain-containing protein 2-B (lmbrd2b), partial CSB-YP754189DIL
CSB-EP754189DIL
CSB-BP754189DIL
CSB-MP754189DIL
CSB-EP754189DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis BRISC complex subunit Abro1 (fam175b) CSB-YP754190XBF
CSB-EP754190XBF
CSB-BP754190XBF
CSB-MP754190XBF
CSB-EP754190XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein BANP (banp), partial CSB-YP754191XBF
CSB-EP754191XBF
CSB-BP754191XBF
CSB-MP754191XBF
CSB-EP754191XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 (Inppl1), partial CSB-YP754192MO
CSB-EP754192MO
CSB-BP754192MO
CSB-MP754192MO
CSB-EP754192MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) CSB-YP754193HU
CSB-EP754193HU
CSB-BP754193HU
CSB-MP754193HU
CSB-EP754193HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (Smchd1), partial CSB-YP754194MO
CSB-EP754194MO
CSB-BP754194MO
CSB-MP754194MO
CSB-EP754194MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 60S ribosomal protein L19 (rpl19) CSB-YP754195DIL
CSB-EP754195DIL
CSB-BP754195DIL
CSB-MP754195DIL
CSB-EP754195DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio LysM and putative peptidoglycan-binding domain-containing protein 4 (lysmd4), partial CSB-YP754196DIL
CSB-EP754196DIL
CSB-BP754196DIL
CSB-MP754196DIL
CSB-EP754196DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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