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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic (ndhG), partial CSB-YP754619AIQ1
CSB-EP754619AIQ1
CSB-BP754619AIQ1
CSB-MP754619AIQ1
CSB-EP754619AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP754620AIQ
CSB-EP754620AIQ
CSB-BP754620AIQ
CSB-MP754620AIQ
CSB-EP754620AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein H (psbH), partial CSB-YP754621AIQ1
CSB-EP754621AIQ1
CSB-BP754621AIQ1
CSB-MP754621AIQ1
CSB-EP754621AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I reaction center subunit IX (psaJ), partial CSB-YP754622AIQ
CSB-EP754622AIQ
CSB-BP754622AIQ
CSB-MP754622AIQ
CSB-EP754622AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroplast envelope membrane protein (cemA), partial CSB-YP754623AIQ1
CSB-EP754623AIQ1
CSB-BP754623AIQ1
CSB-MP754623AIQ1
CSB-EP754623AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP754624AIQ
CSB-EP754624AIQ
CSB-BP754624AIQ
CSB-MP754624AIQ
CSB-EP754624AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP754625AIQ
CSB-EP754625AIQ
CSB-BP754625AIQ
CSB-MP754625AIQ
CSB-EP754625AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S16, chloroplastic (rps16) CSB-YP754626AIQ
CSB-EP754626AIQ
CSB-BP754626AIQ
CSB-MP754626AIQ
CSB-EP754626AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasypus kappleri NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP754627DCAP
CSB-EP754627DCAP
CSB-BP754627DCAP
CSB-MP754627DCAP
CSB-EP754627DCAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Talanin (ZNF365) CSB-YP754628HU
CSB-EP754628HU
CSB-BP754628HU
CSB-MP754628HU
CSB-EP754628HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Secreted phosphoprotein 24 (SPP2) CSB-YP754629CH
CSB-EP754629CH
CSB-BP754629CH
CSB-MP754629CH
CSB-EP754629CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes vulpes Calcium-binding tyrosine phosphorylation-regulated protein (CABYR) CSB-YP754630VFW
CSB-EP754630VFW
CSB-BP754630VFW
CSB-MP754630VFW
CSB-EP754630VFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C10orf71 (C10orf71), partial CSB-YP754631HU
CSB-EP754631HU
CSB-BP754631HU
CSB-MP754631HU
CSB-EP754631HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP754632EXI
CSB-EP754632EXI
CSB-BP754632EXI
CSB-MP754632EXI
CSB-EP754632EXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig cAMP-regulated phosphoprotein 19 (ARPP19) CSB-YP754633PI
CSB-EP754633PI
CSB-BP754633PI
CSB-MP754633PI
CSB-EP754633PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Sf6 Probable excisionase hkaC (18) CSB-YP754634EDT
CSB-EP754634EDT
CSB-BP754634EDT
CSB-MP754634EDT
CSB-EP754634EDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maturase K (matK), partial CSB-YP754635LCAX
CSB-EP754635LCAX
CSB-BP754635LCAX
CSB-MP754635LCAX
CSB-EP754635LCAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Phosducin-like protein 2 (phlp2) CSB-YP754636DKK
CSB-EP754636DKK
CSB-BP754636DKK
CSB-MP754636DKK
CSB-EP754636DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Ciliary neurotrophic factor receptor subunit alpha (CNTFR) CSB-YP754637DO
CSB-EP754637DO
CSB-BP754637DO
CSB-MP754637DO
CSB-EP754637DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpestes ichneumon Cytochrome b (MT-CYB), partial CSB-YP754638HGI
CSB-EP754638HGI
CSB-BP754638HGI
CSB-MP754638HGI
CSB-EP754638HGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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