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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neurospora crassa Inorganic pyrophosphatase (ipp-1) CSB-YP754017NHA
CSB-EP754017NHA
CSB-BP754017NHA
CSB-MP754017NHA
CSB-EP754017NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE61 (ppe61) CSB-YP754018MVZ
CSB-EP754018MVZ
CSB-BP754018MVZ
CSB-MP754018MVZ
CSB-EP754018MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE11 (ppe11), partial CSB-YP754019MVZ
CSB-EP754019MVZ
CSB-BP754019MVZ
CSB-MP754019MVZ
CSB-EP754019MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HLA class I histocompatibility antigen protein P5 (HCP5) CSB-YP754020HU
CSB-EP754020HU
CSB-BP754020HU
CSB-MP754020HU
CSB-EP754020HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 (OGFOD2) CSB-YP754021HU
CSB-EP754021HU
CSB-BP754021HU
CSB-MP754021HU
CSB-EP754021HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Putative nickel-responsive regulator (RPA4756) CSB-YP754022RLU
CSB-EP754022RLU
CSB-BP754022RLU
CSB-MP754022RLU
CSB-EP754022RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP754023RLU
CSB-EP754023RLU
CSB-BP754023RLU
CSB-MP754023RLU
CSB-EP754023RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protein SlyX homolog (slyX) CSB-YP754024RLU
CSB-EP754024RLU
CSB-BP754024RLU
CSB-MP754024RLU
CSB-EP754024RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP754025RLU1
CSB-EP754025RLU1
CSB-BP754025RLU1
CSB-MP754025RLU1
CSB-EP754025RLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0337 protein RPA4217 (RPA4217) CSB-YP754026RLU
CSB-EP754026RLU
CSB-BP754026RLU
CSB-MP754026RLU
CSB-EP754026RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Urease subunit gamma (ureA) CSB-YP754027RLU
CSB-EP754027RLU
CSB-BP754027RLU
CSB-MP754027RLU
CSB-EP754027RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Trans-aconitate 2-methyltransferase (tam) CSB-YP754028RLU
CSB-EP754028RLU
CSB-BP754028RLU
CSB-MP754028RLU
CSB-EP754028RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP754029RLU
CSB-EP754029RLU
CSB-BP754029RLU
CSB-MP754029RLU
CSB-EP754029RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Threonine--tRNA ligase (thrS), partial CSB-YP754030RLU
CSB-EP754030RLU
CSB-BP754030RLU
CSB-MP754030RLU
CSB-EP754030RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L10 (rplJ) CSB-YP754031RLU
CSB-EP754031RLU
CSB-BP754031RLU
CSB-MP754031RLU
CSB-EP754031RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S10 (rpsJ) CSB-YP754032RLU
CSB-EP754032RLU
CSB-BP754032RLU
CSB-MP754032RLU
CSB-EP754032RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S17 (rpsQ) CSB-YP754033RLU
CSB-EP754033RLU
CSB-BP754033RLU
CSB-MP754033RLU
CSB-EP754033RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L17 (rplQ) CSB-YP754034RLU
CSB-EP754034RLU
CSB-BP754034RLU
CSB-MP754034RLU
CSB-EP754034RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP-dependent Clp protease adapter protein ClpS 2 (clpS2) CSB-YP754035RLU
CSB-EP754035RLU
CSB-BP754035RLU
CSB-MP754035RLU
CSB-EP754035RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S6 (rpsF) CSB-YP754036RLU
CSB-EP754036RLU
CSB-BP754036RLU
CSB-MP754036RLU
CSB-EP754036RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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