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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Ribonuclease HII (rnhB) CSB-YP752895EYP
CSB-EP752895EYP
CSB-BP752895EYP
CSB-MP752895EYP
CSB-EP752895EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP752896EYP
CSB-EP752896EYP
CSB-BP752896EYP
CSB-MP752896EYP
CSB-EP752896EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP752898EYP
CSB-EP752898EYP
CSB-BP752898EYP
CSB-MP752898EYP
CSB-EP752898EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribonuclease Z (rnz) CSB-YP752899EYP
CSB-EP752899EYP
CSB-BP752899EYP
CSB-MP752899EYP
CSB-EP752899EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Tyrosine--tRNA ligase (tyrS) CSB-YP752900EYP
CSB-EP752900EYP
CSB-BP752900EYP
CSB-MP752900EYP
CSB-EP752900EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 (murA1) CSB-YP752901EYP
CSB-EP752901EYP
CSB-BP752901EYP
CSB-MP752901EYP
CSB-EP752901EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable inorganic polyphosphate/ATP-NAD kinase 2 (ppnK2) CSB-YP752902EYP
CSB-EP752902EYP
CSB-BP752902EYP
CSB-MP752902EYP
CSB-EP752902EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP752903EYP
CSB-EP752903EYP
CSB-BP752903EYP
CSB-MP752903EYP
CSB-EP752903EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Divinyl chlorophyll a/b light-harvesting protein pcbH (pcbH), partial CSB-YP752904EYP1
CSB-EP752904EYP1
CSB-BP752904EYP1
CSB-MP752904EYP1
CSB-EP752904EYP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP752905EYP
CSB-EP752905EYP
CSB-BP752905EYP
CSB-MP752905EYP
CSB-EP752905EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Tryptophan--tRNA ligase (trpS) CSB-YP752906EYP
CSB-EP752906EYP
CSB-BP752906EYP
CSB-MP752906EYP
CSB-EP752906EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-dehydroquinate synthase (aroB) CSB-YP752907EYP
CSB-EP752907EYP
CSB-BP752907EYP
CSB-MP752907EYP
CSB-EP752907EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Uncharacterized RNA methyltransferase Pro_0965 (Pro_0965) CSB-YP752908EYP
CSB-EP752908EYP
CSB-BP752908EYP
CSB-MP752908EYP
CSB-EP752908EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chaperone protein dnaK1 (dnaK1), partial CSB-YP752909EYP
CSB-EP752909EYP
CSB-BP752909EYP
CSB-MP752909EYP
CSB-EP752909EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Divinyl chlorophyll a/b light-harvesting protein pcbG (pcbG), partial CSB-YP752910EYP1
CSB-EP752910EYP1
CSB-BP752910EYP1
CSB-MP752910EYP1
CSB-EP752910EYP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamyl-tRNA reductase (hemA) CSB-YP752911EYP
CSB-EP752911EYP
CSB-BP752911EYP
CSB-MP752911EYP
CSB-EP752911EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center Psb28 protein (psb28) CSB-YP752912EYP
CSB-EP752912EYP
CSB-BP752912EYP
CSB-MP752912EYP
CSB-EP752912EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S15 (rpsO) CSB-YP752913EYP
CSB-EP752913EYP
CSB-BP752913EYP
CSB-MP752913EYP
CSB-EP752913EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP752914EYP
CSB-EP752914EYP
CSB-BP752914EYP
CSB-MP752914EYP
CSB-EP752914EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ferrochelatase (hemH) CSB-YP752915EYP
CSB-EP752915EYP
CSB-BP752915EYP
CSB-MP752915EYP
CSB-EP752915EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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