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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter sp. Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824EIZ
CSB-EP005824EIZ
CSB-BP005824EIZ
CSB-MP005824EIZ
CSB-EP005824EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Ferrochelatase (hemH) CSB-YP008579EIZ
CSB-EP008579EIZ
CSB-BP008579EIZ
CSB-MP008579EIZ
CSB-EP008579EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. tRNA pseudouridine synthase A (truA) CSB-YP019086EIZ
CSB-EP019086EIZ
CSB-BP019086EIZ
CSB-MP019086EIZ
CSB-EP019086EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 3-ketoacyl-CoA thiolase (fadI), partial CSB-YP001117EIZ
CSB-EP001117EIZ
CSB-BP001117EIZ
CSB-MP001117EIZ
CSB-EP001117EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Serine hydroxymethyltransferase (glyA) CSB-YP021274EIZ
CSB-EP021274EIZ
CSB-BP021274EIZ
CSB-MP021274EIZ
CSB-EP021274EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. GTPase Era (era) CSB-YP007759EIZ
CSB-EP007759EIZ
CSB-BP007759EIZ
CSB-MP007759EIZ
CSB-EP007759EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. N-acetylneuraminate lyase (nanA) CSB-YP015994EIZ
CSB-EP015994EIZ
CSB-BP015994EIZ
CSB-MP015994EIZ
CSB-EP015994EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Triosephosphate isomerase (tpiA) CSB-YP024102EIZ
CSB-EP024102EIZ
CSB-BP024102EIZ
CSB-MP024102EIZ
CSB-EP024102EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658PZU
CSB-EP001658PZU
CSB-BP001658PZU
CSB-MP001658PZU
CSB-EP001658PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Transcription initiation factor IIB (tfb) CSB-YP010001PZU
CSB-EP010001PZU
CSB-BP010001PZU
CSB-MP010001PZU
CSB-EP010001PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP synthase subunit a (atpB), partial CSB-YP015070RLG1
CSB-EP015070RLG1
CSB-BP015070RLG1
CSB-MP015070RLG1
CSB-EP015070RLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA pseudouridine synthase A (truA) CSB-YP019086RLG
CSB-EP019086RLG
CSB-BP019086RLG
CSB-MP019086RLG
CSB-EP019086RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Thymidylate synthase (thyA) CSB-YP025393RLG
CSB-EP025393RLG
CSB-BP025393RLG
CSB-MP025393RLG
CSB-EP025393RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA dimethylallyltransferase (miaA) CSB-YP024529RLG
CSB-EP024529RLG
CSB-BP024529RLG
CSB-MP024529RLG
CSB-EP024529RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493RLG
CSB-EP009493RLG
CSB-BP009493RLG
CSB-MP009493RLG
CSB-EP009493RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribonuclease 3 (rnc) CSB-YP019809RLG
CSB-EP019809RLG
CSB-BP019809RLG
CSB-MP019809RLG
CSB-EP019809RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Methionyl-tRNA formyltransferase (fmt) CSB-YP015150STD
CSB-EP015150STD
CSB-BP015150STD
CSB-MP015150STD
CSB-EP015150STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Serine hydroxymethyltransferase (glyA) CSB-YP021274CVX
CSB-EP021274CVX
CSB-BP021274CVX
CSB-MP021274CVX
CSB-EP021274CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Peptide deformylase (def) CSB-YP017707PZM
CSB-EP017707PZM
CSB-BP017707PZM
CSB-MP017707PZM
CSB-EP017707PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis ATP synthase subunit a (atpB), partial CSB-YP015070YAL1
CSB-EP015070YAL1
CSB-BP015070YAL1
CSB-MP015070YAL1
CSB-EP015070YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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