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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrobaculum arsenaticum Probable imidazolonepropionase (hutI) CSB-YP001659PZU
CSB-EP001659PZU
CSB-BP001659PZU
CSB-MP001659PZU
CSB-EP001659PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Ribosomal RNA small subunit methyltransferase Nep1 (nep1) CSB-YP007636PZU
CSB-EP007636PZU
CSB-BP007636PZU
CSB-MP007636PZU
CSB-EP007636PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582RLG
CSB-EP009582RLG
CSB-BP009582RLG
CSB-MP009582RLG
CSB-EP009582RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Ribosome-recycling factor (frr) CSB-YP014941RLG
CSB-EP014941RLG
CSB-BP014941RLG
CSB-MP014941RLG
CSB-EP014941RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP synthase subunit alpha (atpA), partial CSB-YP002344RLG
CSB-EP002344RLG
CSB-BP002344RLG
CSB-MP002344RLG
CSB-EP002344RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Elongation factor Tu (tuf1) CSB-YP025342RLG
CSB-EP025342RLG
CSB-BP025342RLG
CSB-MP025342RLG
CSB-EP025342RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Argininosuccinate synthase (argG) CSB-YP002234RLG
CSB-EP002234RLG
CSB-BP002234RLG
CSB-MP002234RLG
CSB-EP002234RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Translation initiation factor IF-2 (infB), partial CSB-YP015164RLG
CSB-EP015164RLG
CSB-BP015164RLG
CSB-MP015164RLG
CSB-EP015164RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribosome-recycling factor (frr) CSB-YP014941STD
CSB-EP014941STD
CSB-BP014941STD
CSB-MP014941STD
CSB-EP014941STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Translation initiation factor IF-2 (infB), partial CSB-YP015164STD
CSB-EP015164STD
CSB-BP015164STD
CSB-MP015164STD
CSB-EP015164STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica ATP synthase subunit delta (atpH) CSB-YP002355STD
CSB-EP002355STD
CSB-BP002355STD
CSB-MP002355STD
CSB-EP002355STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Elongation factor G (fusA), partial CSB-YP009373STD
CSB-EP009373STD
CSB-BP009373STD
CSB-MP009373STD
CSB-EP009373STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosome-recycling factor (frr) CSB-YP014941YAL
CSB-EP014941YAL
CSB-BP014941YAL
CSB-MP014941YAL
CSB-EP014941YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Phosphoserine aminotransferase (serC) CSB-YP018838YAL
CSB-EP018838YAL
CSB-BP018838YAL
CSB-MP018838YAL
CSB-EP018838YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Enolase-phosphatase E1 (mtnC) CSB-YP007674YAL
CSB-EP007674YAL
CSB-BP007674YAL
CSB-MP007674YAL
CSB-EP007674YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Malate dehydrogenase (mdh) CSB-YP013623YAL
CSB-EP013623YAL
CSB-BP013623YAL
CSB-MP013623YAL
CSB-EP013623YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA dimethylallyltransferase (miaA) CSB-YP024529YAL
CSB-EP024529YAL
CSB-BP024529YAL
CSB-MP024529YAL
CSB-EP024529YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis L-threonine 3-dehydrogenase (tdh) CSB-YP023350YAL
CSB-EP023350YAL
CSB-BP023350YAL
CSB-MP023350YAL
CSB-EP023350YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Kynureninase (BNA5) CSB-YP012703MPM
CSB-EP012703MPM
CSB-BP012703MPM
CSB-MP012703MPM
CSB-EP012703MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodelphis domestica Tuftelin-interacting protein 11 (TFIP11), partial CSB-YP023435MUE
CSB-EP023435MUE
CSB-BP023435MUE
CSB-MP023435MUE
CSB-EP023435MUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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