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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Haemophilus ducreyi Apolipoprotein N-acyltransferase (lnt), partial CSB-YP752996HSY
CSB-EP752996HSY
CSB-BP752996HSY
CSB-MP752996HSY
CSB-EP752996HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Bifunctional protein FolD (folD) CSB-YP752997HSY
CSB-EP752997HSY
CSB-BP752997HSY
CSB-MP752997HSY
CSB-EP752997HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Protein grpE (grpE) CSB-YP752998HSY
CSB-EP752998HSY
CSB-BP752998HSY
CSB-MP752998HSY
CSB-EP752998HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Primosomal replication protein n (priB) CSB-YP752999HSY
CSB-EP752999HSY
CSB-BP752999HSY
CSB-MP752999HSY
CSB-EP752999HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Protein ProQ homolog (proQ) CSB-YP753000HSY
CSB-EP753000HSY
CSB-BP753000HSY
CSB-MP753000HSY
CSB-EP753000HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP753001HSY1
CSB-EP753001HSY1
CSB-BP753001HSY1
CSB-MP753001HSY1
CSB-EP753001HSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Peptide chain release factor 1 (prfA) CSB-YP753002HSY
CSB-EP753002HSY
CSB-BP753002HSY
CSB-MP753002HSY
CSB-EP753002HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Adenylate kinase (adk) CSB-YP753003HSY
CSB-EP753003HSY
CSB-BP753003HSY
CSB-MP753003HSY
CSB-EP753003HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP753004HSY
CSB-EP753004HSY
CSB-BP753004HSY
CSB-MP753004HSY
CSB-EP753004HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 50S ribosomal protein L34 (rpmH) CSB-YP753005HSY
CSB-EP753005HSY
CSB-BP753005HSY
CSB-MP753005HSY
CSB-EP753005HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi UPF0758 protein HD_0732 (HD_0732) CSB-YP753006HSY
CSB-EP753006HSY
CSB-BP753006HSY
CSB-MP753006HSY
CSB-EP753006HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP753007HSY
CSB-EP753007HSY
CSB-BP753007HSY
CSB-MP753007HSY
CSB-EP753007HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP753008HSY
CSB-EP753008HSY
CSB-BP753008HSY
CSB-MP753008HSY
CSB-EP753008HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Ribonuclease T (rnt) CSB-YP753009HSY
CSB-EP753009HSY
CSB-BP753009HSY
CSB-MP753009HSY
CSB-EP753009HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Chaperone protein ClpB (clpB), partial CSB-YP753010HSY
CSB-EP753010HSY
CSB-BP753010HSY
CSB-MP753010HSY
CSB-EP753010HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi LexA repressor (lexA) CSB-YP753011HSY
CSB-EP753011HSY
CSB-BP753011HSY
CSB-MP753011HSY
CSB-EP753011HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP753012HSY
CSB-EP753012HSY
CSB-BP753012HSY
CSB-MP753012HSY
CSB-EP753012HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Electron transport complex protein RnfE (rnfE), partial CSB-YP753013HSY1
CSB-EP753013HSY1
CSB-BP753013HSY1
CSB-MP753013HSY1
CSB-EP753013HSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Na (+)-translocating NADH-quinone reductase subunit E (nqrE), partial CSB-YP753014HSY1
CSB-EP753014HSY1
CSB-BP753014HSY1
CSB-MP753014HSY1
CSB-EP753014HSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Sulfurtransferase TusA homolog (tusA) CSB-YP753015HSY
CSB-EP753015HSY
CSB-BP753015HSY
CSB-MP753015HSY
CSB-EP753015HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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