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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Protochlamydia amoebophila (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP753937PAAD
CSB-EP753937PAAD
CSB-BP753937PAAD
CSB-MP753937PAAD
CSB-EP753937PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Recombination protein RecR (recR) CSB-YP753938PAAD
CSB-EP753938PAAD
CSB-BP753938PAAD
CSB-MP753938PAAD
CSB-EP753938PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila ATP synthase subunit a (atpB), partial CSB-YP753939PAAD1
CSB-EP753939PAAD1
CSB-BP753939PAAD1
CSB-MP753939PAAD1
CSB-EP753939PAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila ATP synthase epsilon chain (atpC) CSB-YP753940PAAD
CSB-EP753940PAAD
CSB-BP753940PAAD
CSB-MP753940PAAD
CSB-EP753940PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Alanine--tRNA ligase (alaS), partial CSB-YP753941PAAD
CSB-EP753941PAAD
CSB-BP753941PAAD
CSB-MP753941PAAD
CSB-EP753941PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila GTPase Der (der) CSB-YP753942PAAD
CSB-EP753942PAAD
CSB-BP753942PAAD
CSB-MP753942PAAD
CSB-EP753942PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Phosphate acyltransferase (plsX) CSB-YP753943PAAD
CSB-EP753943PAAD
CSB-BP753943PAAD
CSB-MP753943PAAD
CSB-EP753943PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP753944PAAD
CSB-EP753944PAAD
CSB-BP753944PAAD
CSB-MP753944PAAD
CSB-EP753944PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Chromosomal replication initiator protein DnaA 1 (dnaA1) CSB-YP753945PAAD
CSB-EP753945PAAD
CSB-BP753945PAAD
CSB-MP753945PAAD
CSB-EP753945PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Demethylmenaquinone methyltransferase (ubiE) CSB-YP753946PAAD
CSB-EP753946PAAD
CSB-BP753946PAAD
CSB-MP753946PAAD
CSB-EP753946PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Glucose-6-phosphate isomerase (pgi), partial CSB-YP753947PAAD
CSB-EP753947PAAD
CSB-BP753947PAAD
CSB-MP753947PAAD
CSB-EP753947PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribosome-binding factor A (rbfA) CSB-YP753948PAAD
CSB-EP753948PAAD
CSB-BP753948PAAD
CSB-MP753948PAAD
CSB-EP753948PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L20 (rplT) CSB-YP753949PAAD
CSB-EP753949PAAD
CSB-BP753949PAAD
CSB-MP753949PAAD
CSB-EP753949PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L19 (rplS) CSB-YP753950PAAD
CSB-EP753950PAAD
CSB-BP753950PAAD
CSB-MP753950PAAD
CSB-EP753950PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S15 (rpsO) CSB-YP753951PAAD
CSB-EP753951PAAD
CSB-BP753951PAAD
CSB-MP753951PAAD
CSB-EP753951PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP753952PAAD
CSB-EP753952PAAD
CSB-BP753952PAAD
CSB-MP753952PAAD
CSB-EP753952PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L1 (rplA) CSB-YP753953PAAD
CSB-EP753953PAAD
CSB-BP753953PAAD
CSB-MP753953PAAD
CSB-EP753953PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP753954PAAD
CSB-EP753954PAAD
CSB-BP753954PAAD
CSB-MP753954PAAD
CSB-EP753954PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Isoleucine--tRNA ligase (ileS), partial CSB-YP753955PAAD
CSB-EP753955PAAD
CSB-BP753955PAAD
CSB-MP753955PAAD
CSB-EP753955PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Methionine import ATP-binding protein MetN (metN) CSB-YP753956PAAD
CSB-EP753956PAAD
CSB-BP753956PAAD
CSB-MP753956PAAD
CSB-EP753956PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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