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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella pertussis Chaperone protein DnaK (dnaK), partial CSB-YP760173BUA
CSB-EP760173BUA
CSB-BP760173BUA
CSB-MP760173BUA
CSB-EP760173BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP760174BUA
CSB-EP760174BUA
CSB-BP760174BUA
CSB-MP760174BUA
CSB-EP760174BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Malate dehydrogenase (mdh) CSB-YP760175BUA
CSB-EP760175BUA
CSB-BP760175BUA
CSB-MP760175BUA
CSB-EP760175BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP760176BUA
CSB-EP760176BUA
CSB-BP760176BUA
CSB-MP760176BUA
CSB-EP760176BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Histidine--tRNA ligase (hisS) CSB-YP760177BUA
CSB-EP760177BUA
CSB-BP760177BUA
CSB-MP760177BUA
CSB-EP760177BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP760178BUA
CSB-EP760178BUA
CSB-BP760178BUA
CSB-MP760178BUA
CSB-EP760178BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Probable L-aspartate dehydrogenase 2 (nadX2) CSB-YP760179BUA
CSB-EP760179BUA
CSB-BP760179BUA
CSB-MP760179BUA
CSB-EP760179BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Ribosome maturation factor RimM (rimM) CSB-YP760180BUA
CSB-EP760180BUA
CSB-BP760180BUA
CSB-MP760180BUA
CSB-EP760180BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Isoleucine--tRNA ligase (ileS), partial CSB-YP760181BUA
CSB-EP760181BUA
CSB-BP760181BUA
CSB-MP760181BUA
CSB-EP760181BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Guanylate kinase (gmk) CSB-YP760182BUA
CSB-EP760182BUA
CSB-BP760182BUA
CSB-MP760182BUA
CSB-EP760182BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Chorismate synthase (aroC) CSB-YP760183BUA
CSB-EP760183BUA
CSB-BP760183BUA
CSB-MP760183BUA
CSB-EP760183BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Undecaprenyl pyrophosphate synthase (uppS) CSB-YP760184BUA
CSB-EP760184BUA
CSB-BP760184BUA
CSB-MP760184BUA
CSB-EP760184BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 3-isopropylmalate dehydratase small subunit 1 (leuD1) CSB-YP760185BUA
CSB-EP760185BUA
CSB-BP760185BUA
CSB-MP760185BUA
CSB-EP760185BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Translation initiation factor IF-2 (infB), partial CSB-YP760186BUA
CSB-EP760186BUA
CSB-BP760186BUA
CSB-MP760186BUA
CSB-EP760186BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 50S ribosomal protein L31 type B (rpmE2) CSB-YP760187BUA
CSB-EP760187BUA
CSB-BP760187BUA
CSB-MP760187BUA
CSB-EP760187BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Peptide chain release factor 2 (prfB) CSB-YP760188BUA
CSB-EP760188BUA
CSB-BP760188BUA
CSB-MP760188BUA
CSB-EP760188BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP760189BUA
CSB-EP760189BUA
CSB-BP760189BUA
CSB-MP760189BUA
CSB-EP760189BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Bifunctional protein FolD 1 (folD1) CSB-YP760190BUA
CSB-EP760190BUA
CSB-BP760190BUA
CSB-MP760190BUA
CSB-EP760190BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP760191BUA
CSB-EP760191BUA
CSB-BP760191BUA
CSB-MP760191BUA
CSB-EP760191BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Glutamate 5-kinase (proB) CSB-YP760192BUA
CSB-EP760192BUA
CSB-BP760192BUA
CSB-MP760192BUA
CSB-EP760192BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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