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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Delta-hemolysin (hld) CSB-YP760574SKV
CSB-EP760574SKV
CSB-BP760574SKV
CSB-MP760574SKV
CSB-EP760574SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein map (map), partial CSB-YP760575SKV
CSB-EP760575SKV
CSB-BP760575SKV
CSB-MP760575SKV
CSB-EP760575SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0316 protein SAR2004 (SAR2004), partial CSB-YP760576SKV1
CSB-EP760576SKV1
CSB-BP760576SKV1
CSB-MP760576SKV1
CSB-EP760576SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Sodium/proline symporter (putP), partial CSB-YP760577SKV
CSB-EP760577SKV
CSB-BP760577SKV
CSB-MP760577SKV
CSB-EP760577SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0421 protein SAR1980 (SAR1980), partial CSB-YP760578SKV1
CSB-EP760578SKV1
CSB-BP760578SKV1
CSB-MP760578SKV1
CSB-EP760578SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative multidrug export ATP-binding/permease protein SAR1956 (SAR1956), partial CSB-YP760579SKV1
CSB-EP760579SKV1
CSB-BP760579SKV1
CSB-MP760579SKV1
CSB-EP760579SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0754 membrane protein SAR1937 (SAR1937), partial CSB-YP760580SKV1
CSB-EP760580SKV1
CSB-BP760580SKV1
CSB-MP760580SKV1
CSB-EP760580SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ferrochelatase (hemH) CSB-YP760581SKV
CSB-EP760581SKV
CSB-BP760581SKV
CSB-MP760581SKV
CSB-EP760581SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP760582SKV
CSB-EP760582SKV
CSB-BP760582SKV
CSB-MP760582SKV
CSB-EP760582SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP760583SKV
CSB-EP760583SKV
CSB-BP760583SKV
CSB-MP760583SKV
CSB-EP760583SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative universal stress protein SAR1788 (SAR1788) CSB-YP760584SKV
CSB-EP760584SKV
CSB-BP760584SKV
CSB-MP760584SKV
CSB-EP760584SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Translation initiation factor IF-3 (infC) CSB-YP760585SKV
CSB-EP760585SKV
CSB-BP760585SKV
CSB-MP760585SKV
CSB-EP760585SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Valine--tRNA ligase (valS), partial CSB-YP760586SKV
CSB-EP760586SKV
CSB-BP760586SKV
CSB-MP760586SKV
CSB-EP760586SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP760587SKV
CSB-EP760587SKV
CSB-BP760587SKV
CSB-MP760587SKV
CSB-EP760587SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S20 (rpsT) CSB-YP760588SKV
CSB-EP760588SKV
CSB-BP760588SKV
CSB-MP760588SKV
CSB-EP760588SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S21 (rpsU) CSB-YP760589SKV
CSB-EP760589SKV
CSB-BP760589SKV
CSB-MP760589SKV
CSB-EP760589SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Sensor protein srrB (srrB), partial CSB-YP760590SKV
CSB-EP760590SKV
CSB-BP760590SKV
CSB-MP760590SKV
CSB-EP760590SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP760591SKV
CSB-EP760591SKV
CSB-BP760591SKV
CSB-MP760591SKV
CSB-EP760591SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction resolvase recU (recU) CSB-YP760592SKV
CSB-EP760592SKV
CSB-BP760592SKV
CSB-MP760592SKV
CSB-EP760592SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Thymidylate synthase (thyA) CSB-YP760593SKV
CSB-EP760593SKV
CSB-BP760593SKV
CSB-MP760593SKV
CSB-EP760593SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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