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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 30S ribosomal protein S2 (rpsB) CSB-YP760253BIK
CSB-EP760253BIK
CSB-BP760253BIK
CSB-MP760253BIK
CSB-EP760253BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP760254BIK
CSB-EP760254BIK
CSB-BP760254BIK
CSB-MP760254BIK
CSB-EP760254BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP760255BIK
CSB-EP760255BIK
CSB-BP760255BIK
CSB-MP760255BIK
CSB-EP760255BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (BPP1139) CSB-YP760256BIK
CSB-EP760256BIK
CSB-BP760256BIK
CSB-MP760256BIK
CSB-EP760256BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP760257BIK
CSB-EP760257BIK
CSB-BP760257BIK
CSB-MP760257BIK
CSB-EP760257BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP760258BIK
CSB-EP760258BIK
CSB-BP760258BIK
CSB-MP760258BIK
CSB-EP760258BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UPF0246 protein BB3890 (BB3890) CSB-YP760259BTY
CSB-EP760259BTY
CSB-BP760259BTY
CSB-MP760259BTY
CSB-EP760259BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP760260BTY
CSB-EP760260BTY
CSB-BP760260BTY
CSB-MP760260BTY
CSB-EP760260BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Tryptophan synthase beta chain (trpB) CSB-YP760261BTY
CSB-EP760261BTY
CSB-BP760261BTY
CSB-MP760261BTY
CSB-EP760261BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Acyl carrier protein (acpP) CSB-YP760262BTY
CSB-EP760262BTY
CSB-BP760262BTY
CSB-MP760262BTY
CSB-EP760262BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Cell division protein FtsB (ftsB), partial CSB-YP760263BTY
CSB-EP760263BTY
CSB-BP760263BTY
CSB-MP760263BTY
CSB-EP760263BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Acylphosphatase (acyP) CSB-YP760264BTY
CSB-EP760264BTY
CSB-BP760264BTY
CSB-MP760264BTY
CSB-EP760264BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Peptide chain release factor 3 (prfC), partial CSB-YP760265BTY
CSB-EP760265BTY
CSB-BP760265BTY
CSB-MP760265BTY
CSB-EP760265BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Type IV secretion system protein ptlC homolog (ptlC), partial CSB-YP760266BTY
CSB-EP760266BTY
CSB-BP760266BTY
CSB-MP760266BTY
CSB-EP760266BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP760267BTY
CSB-EP760267BTY
CSB-BP760267BTY
CSB-MP760267BTY
CSB-EP760267BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Protoheme IX farnesyltransferase (ctaB), partial CSB-YP760268BTY
CSB-EP760268BTY
CSB-BP760268BTY
CSB-MP760268BTY
CSB-EP760268BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Indole-3-glycerol phosphate synthase (trpC) CSB-YP760269BTY
CSB-EP760269BTY
CSB-BP760269BTY
CSB-MP760269BTY
CSB-EP760269BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica ATP synthase subunit delta (atpH) CSB-YP760270BTY
CSB-EP760270BTY
CSB-BP760270BTY
CSB-MP760270BTY
CSB-EP760270BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Homoserine O-acetyltransferase (metX) CSB-YP760271BTY
CSB-EP760271BTY
CSB-BP760271BTY
CSB-MP760271BTY
CSB-EP760271BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Glycerol kinase (glpK), partial CSB-YP760272BTY
CSB-EP760272BTY
CSB-BP760272BTY
CSB-MP760272BTY
CSB-EP760272BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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