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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Alanine dehydrogenase 2 (ald2) CSB-YP760474SKW
CSB-EP760474SKW
CSB-BP760474SKW
CSB-MP760474SKW
CSB-EP760474SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isocitrate dehydrogenase [NADP] (icd) CSB-YP760475SKW
CSB-EP760475SKW
CSB-BP760475SKW
CSB-MP760475SKW
CSB-EP760475SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamyl-tRNA reductase (hemA) CSB-YP760476SKW
CSB-EP760476SKW
CSB-BP760476SKW
CSB-MP760476SKW
CSB-EP760476SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0735 ACT domain-containing protein SAS1579 (SAS1579) CSB-YP760477SKW
CSB-EP760477SKW
CSB-BP760477SKW
CSB-MP760477SKW
CSB-EP760477SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTP pyrophosphokinase (relA), partial CSB-YP760478SKW
CSB-EP760478SKW
CSB-BP760478SKW
CSB-MP760478SKW
CSB-EP760478SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0297 protein SAS1553 (SAS1553) CSB-YP760479SKW
CSB-EP760479SKW
CSB-BP760479SKW
CSB-MP760479SKW
CSB-EP760479SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uridine kinase (udk) CSB-YP760480SKW
CSB-EP760480SKW
CSB-BP760480SKW
CSB-MP760480SKW
CSB-EP760480SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP760481SKW
CSB-EP760481SKW
CSB-BP760481SKW
CSB-MP760481SKW
CSB-EP760481SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycine--tRNA ligase (glyQS) CSB-YP760482SKW
CSB-EP760482SKW
CSB-BP760482SKW
CSB-MP760482SKW
CSB-EP760482SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 1 (rpmG1) CSB-YP760483SKW
CSB-EP760483SKW
CSB-BP760483SKW
CSB-MP760483SKW
CSB-EP760483SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP760484SKW
CSB-EP760484SKW
CSB-BP760484SKW
CSB-MP760484SKW
CSB-EP760484SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elastin-binding protein ebpS (ebpS), partial CSB-YP760485SKW1
CSB-EP760485SKW1
CSB-BP760485SKW1
CSB-MP760485SKW1
CSB-EP760485SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chorismate synthase (aroC) CSB-YP760486SKW
CSB-EP760486SKW
CSB-BP760486SKW
CSB-MP760486SKW
CSB-EP760486SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction resolvase recU (recU) CSB-YP760487SKW
CSB-EP760487SKW
CSB-BP760487SKW
CSB-MP760487SKW
CSB-EP760487SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Conserved virulence factor C (cvfC) CSB-YP760488SKW
CSB-EP760488SKW
CSB-BP760488SKW
CSB-MP760488SKW
CSB-EP760488SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0346 protein SAS1364 (SAS1364) CSB-YP760489SKW
CSB-EP760489SKW
CSB-BP760489SKW
CSB-MP760489SKW
CSB-EP760489SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SAS1352 (SAS1352) CSB-YP760490SKW
CSB-EP760490SKW
CSB-BP760490SKW
CSB-MP760490SKW
CSB-EP760490SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cold shock protein CspA (cspA) CSB-YP760491SKW
CSB-EP760491SKW
CSB-BP760491SKW
CSB-MP760491SKW
CSB-EP760491SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Conserved virulence factor B (cvfB) CSB-YP760492SKW
CSB-EP760492SKW
CSB-BP760492SKW
CSB-MP760492SKW
CSB-EP760492SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative oligopeptide transport ATP-binding protein oppF2 (oppF2) CSB-YP760493SKW
CSB-EP760493SKW
CSB-BP760493SKW
CSB-MP760493SKW
CSB-EP760493SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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