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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Translation initiation factor IF-1 (infA) CSB-YP760454SKW
CSB-EP760454SKW
CSB-BP760454SKW
CSB-MP760454SKW
CSB-EP760454SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP760455SKW
CSB-EP760455SKW
CSB-BP760455SKW
CSB-MP760455SKW
CSB-EP760455SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginase (arg) CSB-YP760456SKW
CSB-EP760456SKW
CSB-BP760456SKW
CSB-MP760456SKW
CSB-EP760456SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus S-ribosylhomocysteine lyase (luxS) CSB-YP760457SKW
CSB-EP760457SKW
CSB-BP760457SKW
CSB-MP760457SKW
CSB-EP760457SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP760458SKW
CSB-EP760458SKW
CSB-BP760458SKW
CSB-MP760458SKW
CSB-EP760458SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Thymidine kinase (tdk) CSB-YP760459SKW
CSB-EP760459SKW
CSB-BP760459SKW
CSB-MP760459SKW
CSB-EP760459SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit b (atpF), partial CSB-YP760460SKW1
CSB-EP760460SKW1
CSB-BP760460SKW1
CSB-MP760460SKW1
CSB-EP760460SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine--D-alanine ligase (ddl) CSB-YP760461SKW
CSB-EP760461SKW
CSB-BP760461SKW
CSB-MP760461SKW
CSB-EP760461SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Anti-sigma-B factor antagonist (rsbV) CSB-YP760462SKW
CSB-EP760462SKW
CSB-BP760462SKW
CSB-MP760462SKW
CSB-EP760462SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP760463SKW
CSB-EP760463SKW
CSB-BP760463SKW
CSB-MP760463SKW
CSB-EP760463SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable succinyl-diaminopimelate desuccinylase (dapE) CSB-YP760464SKW
CSB-EP760464SKW
CSB-BP760464SKW
CSB-MP760464SKW
CSB-EP760464SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Staphylokinase (sak) CSB-YP760465SKW
CSB-EP760465SKW
CSB-BP760465SKW
CSB-MP760465SKW
CSB-EP760465SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Staphopain A (sspP) CSB-YP760466SKW
CSB-EP760466SKW
CSB-BP760466SKW
CSB-MP760466SKW
CSB-EP760466SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase IV (dinB) CSB-YP760467SKW
CSB-EP760467SKW
CSB-BP760467SKW
CSB-MP760467SKW
CSB-EP760467SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Methionine aminopeptidase (map) CSB-YP760468SKW
CSB-EP760468SKW
CSB-BP760468SKW
CSB-MP760468SKW
CSB-EP760468SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Signal transduction protein TRAP (traP) CSB-YP760469SKW
CSB-EP760469SKW
CSB-BP760469SKW
CSB-MP760469SKW
CSB-EP760469SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splB (splB) CSB-YP760470SKW
CSB-EP760470SKW
CSB-BP760470SKW
CSB-MP760470SKW
CSB-EP760470SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP760471SKW
CSB-EP760471SKW
CSB-BP760471SKW
CSB-MP760471SKW
CSB-EP760471SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP760472SKW
CSB-EP760472SKW
CSB-BP760472SKW
CSB-MP760472SKW
CSB-EP760472SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetoin utilization protein AcuC (acuC) CSB-YP760473SKW
CSB-EP760473SKW
CSB-BP760473SKW
CSB-MP760473SKW
CSB-EP760473SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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