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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bartonella henselae 50S ribosomal protein L10 (rplJ) CSB-YP760413BSG
CSB-EP760413BSG
CSB-BP760413BSG
CSB-MP760413BSG
CSB-EP760413BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP760414BSG
CSB-EP760414BSG
CSB-BP760414BSG
CSB-MP760414BSG
CSB-EP760414BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Bifunctional enzyme IspD/IspF (ispDF) CSB-YP760415BSG
CSB-EP760415BSG
CSB-BP760415BSG
CSB-MP760415BSG
CSB-EP760415BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP760416BSG
CSB-EP760416BSG
CSB-BP760416BSG
CSB-MP760416BSG
CSB-EP760416BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP760417BSG
CSB-EP760417BSG
CSB-BP760417BSG
CSB-MP760417BSG
CSB-EP760417BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae UPF0301 protein BH04450 (BH04450) CSB-YP760418BSG
CSB-EP760418BSG
CSB-BP760418BSG
CSB-MP760418BSG
CSB-EP760418BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP760419BSG
CSB-EP760419BSG
CSB-BP760419BSG
CSB-MP760419BSG
CSB-EP760419BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Adenylosuccinate synthetase (purA) CSB-YP760420BSG
CSB-EP760420BSG
CSB-BP760420BSG
CSB-MP760420BSG
CSB-EP760420BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae UPF0133 protein BH02310 (BH02310) CSB-YP760421BSG
CSB-EP760421BSG
CSB-BP760421BSG
CSB-MP760421BSG
CSB-EP760421BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Gamma-glutamyl phosphate reductase (proA) CSB-YP760422BSG
CSB-EP760422BSG
CSB-BP760422BSG
CSB-MP760422BSG
CSB-EP760422BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 50S ribosomal protein L21 (rplU) CSB-YP760423BSG
CSB-EP760423BSG
CSB-BP760423BSG
CSB-MP760423BSG
CSB-EP760423BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Dihydroxy-acid dehydratase (ilvD), partial CSB-YP760424BSG
CSB-EP760424BSG
CSB-BP760424BSG
CSB-MP760424BSG
CSB-EP760424BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Heat-inducible transcription repressor HrcA (hrcA) CSB-YP760425BSG
CSB-EP760425BSG
CSB-BP760425BSG
CSB-MP760425BSG
CSB-EP760425BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Translation initiation factor IF-3 (infC) CSB-YP760426BSG
CSB-EP760426BSG
CSB-BP760426BSG
CSB-MP760426BSG
CSB-EP760426BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Shikimate dehydrogenase (aroE) CSB-YP760427BSG
CSB-EP760427BSG
CSB-BP760427BSG
CSB-MP760427BSG
CSB-EP760427BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Uridylate kinase (pyrH) CSB-YP760428BSG
CSB-EP760428BSG
CSB-BP760428BSG
CSB-MP760428BSG
CSB-EP760428BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Peptide deformylase (def) CSB-YP760429BSG
CSB-EP760429BSG
CSB-BP760429BSG
CSB-MP760429BSG
CSB-EP760429BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP760430BSG
CSB-EP760430BSG
CSB-BP760430BSG
CSB-MP760430BSG
CSB-EP760430BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Protein nrdI (nrdI) CSB-YP760431BSG
CSB-EP760431BSG
CSB-BP760431BSG
CSB-MP760431BSG
CSB-EP760431BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Probable transcriptional regulatory protein BH14810 (BH14810) CSB-YP760432BSG
CSB-EP760432BSG
CSB-BP760432BSG
CSB-MP760432BSG
CSB-EP760432BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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