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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP760514SKW
CSB-EP760514SKW
CSB-BP760514SKW
CSB-MP760514SKW
CSB-EP760514SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit D1 (mnhD1), partial CSB-YP760515SKW
CSB-EP760515SKW
CSB-BP760515SKW
CSB-MP760515SKW
CSB-EP760515SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0051 protein SAS0788 (SAS0788) CSB-YP760516SKW
CSB-EP760516SKW
CSB-BP760516SKW
CSB-MP760516SKW
CSB-EP760516SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycine cleavage system H protein (gcvH) CSB-YP760517SKW
CSB-EP760517SKW
CSB-BP760517SKW
CSB-MP760517SKW
CSB-EP760517SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative acetyltransferase SAS0727 (SAS0727) CSB-YP760518SKW
CSB-EP760518SKW
CSB-BP760518SKW
CSB-MP760518SKW
CSB-EP760518SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP760519SKW
CSB-EP760519SKW
CSB-BP760519SKW
CSB-MP760519SKW
CSB-EP760519SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative 5' (3')-deoxyribonucleotidase (SAS0690) CSB-YP760520SKW
CSB-EP760520SKW
CSB-BP760520SKW
CSB-MP760520SKW
CSB-EP760520SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidine protein kinase saeS (saeS), partial CSB-YP760521SKW1
CSB-EP760521SKW1
CSB-BP760521SKW1
CSB-MP760521SKW1
CSB-EP760521SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator protein graR (graR) CSB-YP760522SKW
CSB-EP760522SKW
CSB-BP760522SKW
CSB-MP760522SKW
CSB-EP760522SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhE2 (mnhE2), partial CSB-YP760523SKW1
CSB-EP760523SKW1
CSB-BP760523SKW1
CSB-MP760523SKW1
CSB-EP760523SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcriptional regulator sarA (sarA) CSB-YP760524SKW
CSB-EP760524SKW
CSB-BP760524SKW
CSB-MP760524SKW
CSB-EP760524SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphate acetyltransferase (pta) CSB-YP760525SKW
CSB-EP760525SKW
CSB-BP760525SKW
CSB-MP760525SKW
CSB-EP760525SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative proline/betaine transporter (proP), partial CSB-YP760526SKW
CSB-EP760526SKW
CSB-BP760526SKW
CSB-MP760526SKW
CSB-EP760526SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribulokinase (araB), partial CSB-YP760527SKW
CSB-EP760527SKW
CSB-BP760527SKW
CSB-MP760527SKW
CSB-EP760527SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S12 (rpsL) CSB-YP760528SKW
CSB-EP760528SKW
CSB-BP760528SKW
CSB-MP760528SKW
CSB-EP760528SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L10 (rplJ) CSB-YP760529SKW
CSB-EP760529SKW
CSB-BP760529SKW
CSB-MP760529SKW
CSB-EP760529SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Preprotein translocase subunit SecE (secE), partial CSB-YP760530SKW
CSB-EP760530SKW
CSB-BP760530SKW
CSB-MP760530SKW
CSB-EP760530SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydropteroate synthase (folP) CSB-YP760531SKW
CSB-EP760531SKW
CSB-BP760531SKW
CSB-MP760531SKW
CSB-EP760531SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA (Ile)-lysidine synthase (tilS) CSB-YP760532SKW
CSB-EP760532SKW
CSB-BP760532SKW
CSB-MP760532SKW
CSB-EP760532SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP760533SKW
CSB-EP760533SKW
CSB-BP760533SKW
CSB-MP760533SKW
CSB-EP760533SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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