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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Catenin alpha-2 (ctnna2), partial CSB-YP760654XBE
CSB-EP760654XBE
CSB-BP760654XBE
CSB-MP760654XBE
CSB-EP760654XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Peptidyl-prolyl cis-trans isomerase CWC27 homolog (cwc27) CSB-YP760655XBE
CSB-EP760655XBE
CSB-BP760655XBE
CSB-MP760655XBE
CSB-EP760655XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Reprimo-like protein (rprml), partial CSB-YP760656XBE1
CSB-EP760656XBE1
CSB-BP760656XBE1
CSB-MP760656XBE1
CSB-EP760656XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Phospholipase A-2-activating protein (plaa), partial CSB-YP760657XBE
CSB-EP760657XBE
CSB-BP760657XBE
CSB-MP760657XBE
CSB-EP760657XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Alcohol dehydrogenase [NADP (+)] (akr1a1) CSB-YP760658XBE
CSB-EP760658XBE
CSB-BP760658XBE
CSB-MP760658XBE
CSB-EP760658XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Enolase-phosphatase E1 (enoph1) CSB-YP760659DIL
CSB-EP760659DIL
CSB-BP760659DIL
CSB-MP760659DIL
CSB-EP760659DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Putative deoxyribonuclease TATDN1 (tatdn1) CSB-YP760660DIL
CSB-EP760660DIL
CSB-BP760660DIL
CSB-MP760660DIL
CSB-EP760660DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM92A1-B (fam92a1-b) CSB-YP760661XBE
CSB-EP760661XBE
CSB-BP760661XBE
CSB-MP760661XBE
CSB-EP760661XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein canopy homolog 3 (cnpy3) CSB-YP760662XBE
CSB-EP760662XBE
CSB-BP760662XBE
CSB-MP760662XBE
CSB-EP760662XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (c1galt1), partial CSB-YP760663XBE
CSB-EP760663XBE
CSB-BP760663XBE
CSB-MP760663XBE
CSB-EP760663XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger protein 652-A (znf652-a), partial CSB-YP760664XBE
CSB-EP760664XBE
CSB-BP760664XBE
CSB-MP760664XBE
CSB-EP760664XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein MEMO1 (memo1) CSB-YP760665XBE
CSB-EP760665XBE
CSB-BP760665XBE
CSB-MP760665XBE
CSB-EP760665XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein MON2 homolog (mon2), partial CSB-YP760666XBE
CSB-EP760666XBE
CSB-BP760666XBE
CSB-MP760666XBE
CSB-EP760666XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Vacuolar protein sorting-associated protein 29 (vps29) CSB-YP760667XBE
CSB-EP760667XBE
CSB-BP760667XBE
CSB-MP760667XBE
CSB-EP760667XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase KCMF1 (kcmf1) CSB-YP760668XBE
CSB-EP760668XBE
CSB-BP760668XBE
CSB-MP760668XBE
CSB-EP760668XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Uridine-cytidine kinase 1-B (uck1-b) CSB-YP760669XBE
CSB-EP760669XBE
CSB-BP760669XBE
CSB-MP760669XBE
CSB-EP760669XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein kinase TAO2 (taok2), partial CSB-YP760670XBE
CSB-EP760670XBE
CSB-BP760670XBE
CSB-MP760670XBE
CSB-EP760670XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Putative deoxyribonuclease tatdn3-B (tatdn3-b) CSB-YP760671XBE
CSB-EP760671XBE
CSB-BP760671XBE
CSB-MP760671XBE
CSB-EP760671XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Diphthamide biosynthesis protein 1 (dph1) CSB-YP760672XBE
CSB-EP760672XBE
CSB-BP760672XBE
CSB-MP760672XBE
CSB-EP760672XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Rab-like protein 3 (rabl3) CSB-YP760673XBE
CSB-EP760673XBE
CSB-BP760673XBE
CSB-MP760673XBE
CSB-EP760673XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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