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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat Keratin, type I cytoskeletal 17 (Krt17) CSB-YP760794RA
CSB-EP760794RA
CSB-BP760794RA
CSB-MP760794RA
CSB-EP760794RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type I cytoskeletal 10 (Krt10) CSB-YP760795RA
CSB-EP760795RA
CSB-BP760795RA
CSB-MP760795RA
CSB-EP760795RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Reticulon-1-A (rtn1-a), partial CSB-YP760796XBE1
CSB-EP760796XBE1
CSB-BP760796XBE1
CSB-MP760796XBE1
CSB-EP760796XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type II cytoskeletal 1b (Krt77) CSB-YP760797RA
CSB-EP760797RA
CSB-BP760797RA
CSB-MP760797RA
CSB-EP760797RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type II cytoskeletal 72 (Krt72) CSB-YP760798MO
CSB-EP760798MO
CSB-BP760798MO
CSB-MP760798MO
CSB-EP760798MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Testis, prostate and placenta-expressed protein (Tepp) CSB-YP760799MO
CSB-EP760799MO
CSB-BP760799MO
CSB-MP760799MO
CSB-EP760799MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio DnaJ homolog subfamily C member 27 (dnajc27) CSB-YP760800DIL
CSB-EP760800DIL
CSB-BP760800DIL
CSB-MP760800DIL
CSB-EP760800DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein phosphatase 1 regulatory subunit 3B (Ppp1r3b) CSB-YP760801RA
CSB-EP760801RA
CSB-BP760801RA
CSB-MP760801RA
CSB-EP760801RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0361 protein C3orf37 homolog CSB-YP760802XBE
CSB-EP760802XBE
CSB-BP760802XBE
CSB-MP760802XBE
CSB-EP760802XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Rho GTPase-activating protein 19 (arhgap19), partial CSB-YP760803XBE
CSB-EP760803XBE
CSB-BP760803XBE
CSB-MP760803XBE
CSB-EP760803XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Phosphoinositide 3-kinase regulatory subunit 5 (pik3r5), partial CSB-YP760804XBE
CSB-EP760804XBE
CSB-BP760804XBE
CSB-MP760804XBE
CSB-EP760804XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Uncharacterized aarF domain-containing protein kinase 1 (adck1) CSB-YP760805XBE
CSB-EP760805XBE
CSB-BP760805XBE
CSB-MP760805XBE
CSB-EP760805XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Breast cancer anti-estrogen resistance protein 3 homolog (bcar3), partial CSB-YP760806XBE
CSB-EP760806XBE
CSB-BP760806XBE
CSB-MP760806XBE
CSB-EP760806XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis TP53-regulated inhibitor of apoptosis 1-A (triap1-a) CSB-YP760807XBE
CSB-EP760807XBE
CSB-BP760807XBE
CSB-MP760807XBE
CSB-EP760807XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tetratricopeptide repeat protein 35-A (ttc35-a) CSB-YP760808XBE
CSB-EP760808XBE
CSB-BP760808XBE
CSB-MP760808XBE
CSB-EP760808XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Malectin-A (mlec-a), partial CSB-YP760809XBE
CSB-EP760809XBE
CSB-BP760809XBE
CSB-MP760809XBE
CSB-EP760809XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Charged multivesicular body protein 2a (chmp2a) CSB-YP760810XBE
CSB-EP760810XBE
CSB-BP760810XBE
CSB-MP760810XBE
CSB-EP760810XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mediator of RNA polymerase II transcription subunit 10 (med10) CSB-YP760811XBE
CSB-EP760811XBE
CSB-BP760811XBE
CSB-MP760811XBE
CSB-EP760811XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human IQ domain-containing protein E (IQCE) CSB-YP760812HU
CSB-EP760812HU
CSB-BP760812HU
CSB-MP760812HU
CSB-EP760812HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM71E1 (FAM71E1) CSB-YP760813HU
CSB-EP760813HU
CSB-BP760813HU
CSB-MP760813HU
CSB-EP760813HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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