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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Physcomitrella patens subsp. patens ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP761595EVD1
CSB-EP761595EVD1
CSB-BP761595EVD1
CSB-MP761595EVD1
CSB-EP761595EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 50S ribosomal protein L22, chloroplastic (rpl22) CSB-YP761596EVD
CSB-EP761596EVD
CSB-BP761596EVD
CSB-MP761596EVD
CSB-EP761596EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP761597EVD
CSB-EP761597EVD
CSB-BP761597EVD
CSB-MP761597EVD
CSB-EP761597EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP761598EVD
CSB-EP761598EVD
CSB-BP761598EVD
CSB-MP761598EVD
CSB-EP761598EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP761599EVD
CSB-EP761599EVD
CSB-BP761599EVD
CSB-MP761599EVD
CSB-EP761599EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP761600EVD
CSB-EP761600EVD
CSB-BP761600EVD
CSB-MP761600EVD
CSB-EP761600EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP761601EVD
CSB-EP761601EVD
CSB-BP761601EVD
CSB-MP761601EVD
CSB-EP761601EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens NAD (P)H-quinone oxidoreductase subunit K, chloroplastic (ndhK) CSB-YP761602EVD
CSB-EP761602EVD
CSB-BP761602EVD
CSB-MP761602EVD
CSB-EP761602EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoglucanase 19 (Os08g0114200, LOC_Os08g02220) CSB-YP761603OFG
CSB-EP761603OFG
CSB-BP761603OFG
CSB-MP761603OFG
CSB-EP761603OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Lysine-specific histone demethylase 1 homolog 2 (Os08g0143400, LOC_Os08g04780), partial CSB-YP761604OFG
CSB-EP761604OFG
CSB-BP761604OFG
CSB-MP761604OFG
CSB-EP761604OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Germin-like protein 8-6 (Os08g0189500, LOC_Os08g09000) CSB-YP761605OFG
CSB-EP761605OFG
CSB-BP761605OFG
CSB-MP761605OFG
CSB-EP761605OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin response factor 21 (ARF21), partial CSB-YP761606OFG
CSB-EP761606OFG
CSB-BP761606OFG
CSB-MP761606OFG
CSB-EP761606OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os08g0324300 (Os08g0324300, LOC_Os08g23470) CSB-YP761607OFG
CSB-EP761607OFG
CSB-BP761607OFG
CSB-MP761607OFG
CSB-EP761607OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ribose-phosphate pyrophosphokinase 1, chloroplastic (Os02g0127700, LOC_Os02g03540) CSB-YP761608OFG
CSB-EP761608OFG
CSB-BP761608OFG
CSB-MP761608OFG
CSB-EP761608OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative mannan endo-1,4-beta-mannosidase 9 (MAN9) CSB-YP761609OFG
CSB-EP761609OFG
CSB-BP761609OFG
CSB-MP761609OFG
CSB-EP761609OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os07g0679700 (Os07g0679700, LOC_Os07g48200), partial CSB-YP761610OFG
CSB-EP761610OFG
CSB-BP761610OFG
CSB-MP761610OFG
CSB-EP761610OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica COBRA-like protein 6 (BC1L7) CSB-YP761611OFG
CSB-EP761611OFG
CSB-BP761611OFG
CSB-MP761611OFG
CSB-EP761611OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CRS2-associated factor 1, mitochondrial (Os08g0174900, LOC_Os08g07790) CSB-YP761612OFG
CSB-EP761612OFG
CSB-BP761612OFG
CSB-MP761612OFG
CSB-EP761612OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Beta-galactosidase 4 (Os02g0219200, LOC_Os02g12730), partial CSB-YP761613OFG
CSB-EP761613OFG
CSB-BP761613OFG
CSB-MP761613OFG
CSB-EP761613OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-dependent kinase F-4 (CDKF-4) CSB-YP761614OFG
CSB-EP761614OFG
CSB-BP761614OFG
CSB-MP761614OFG
CSB-EP761614OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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