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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii 54S ribosomal protein L4, mitochondrial (MRPL4), partial CSB-YP762216DOT
CSB-EP762216DOT
CSB-BP762216DOT
CSB-MP762216DOT
CSB-EP762216DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal protein L31 (RPL31) CSB-YP762217DOT
CSB-EP762217DOT
CSB-BP762217DOT
CSB-MP762217DOT
CSB-EP762217DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA repair protein RAD59 (RAD59) CSB-YP762218DOT
CSB-EP762218DOT
CSB-BP762218DOT
CSB-MP762218DOT
CSB-EP762218DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RNA exonuclease 4 (REX4) CSB-YP762219DOT
CSB-EP762219DOT
CSB-BP762219DOT
CSB-MP762219DOT
CSB-EP762219DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable trehalase (NTH2), partial CSB-YP762220DOT
CSB-EP762220DOT
CSB-BP762220DOT
CSB-MP762220DOT
CSB-EP762220DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone H3 (HHT1) CSB-YP762221DOT
CSB-EP762221DOT
CSB-BP762221DOT
CSB-MP762221DOT
CSB-EP762221DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable E3 ubiquitin-protein ligase HUL4 (HUL4), partial CSB-YP762222DOT
CSB-EP762222DOT
CSB-BP762222DOT
CSB-MP762222DOT
CSB-EP762222DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable serine/threonine-protein kinase HAL5-like (AEL118C), partial CSB-YP762223DOT
CSB-EP762223DOT
CSB-BP762223DOT
CSB-MP762223DOT
CSB-EP762223DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GPI ethanolamine phosphate transferase 2 (LAS21), partial CSB-YP762224DOT
CSB-EP762224DOT
CSB-BP762224DOT
CSB-MP762224DOT
CSB-EP762224DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Geranylgeranyl pyrophosphate synthase (BTS1) CSB-YP762225DOT
CSB-EP762225DOT
CSB-BP762225DOT
CSB-MP762225DOT
CSB-EP762225DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA-directed RNA polymerase III subunit RPC3 (RPC82), partial CSB-YP762226DOT
CSB-EP762226DOT
CSB-BP762226DOT
CSB-MP762226DOT
CSB-EP762226DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 54S ribosomal protein L51, mitochondrial (MRPL51) CSB-YP762227DOT
CSB-EP762227DOT
CSB-BP762227DOT
CSB-MP762227DOT
CSB-EP762227DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA polymerase epsilon subunit B (DPB2), partial CSB-YP762228DOT
CSB-EP762228DOT
CSB-BP762228DOT
CSB-MP762228DOT
CSB-EP762228DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Sorting nexin-3 (SNX3) CSB-YP762229DOT
CSB-EP762229DOT
CSB-BP762229DOT
CSB-MP762229DOT
CSB-EP762229DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM14 (PAM18), partial CSB-YP762230DOT1
CSB-EP762230DOT1
CSB-BP762230DOT1
CSB-MP762230DOT1
CSB-EP762230DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATPase GET3 (GET3) CSB-YP762231DOT
CSB-EP762231DOT
CSB-BP762231DOT
CSB-MP762231DOT
CSB-EP762231DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein SIP5 (SIP5) CSB-YP762232DOT
CSB-EP762232DOT
CSB-BP762232DOT
CSB-MP762232DOT
CSB-EP762232DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Sterol 24-C-methyltransferase (ERG6) CSB-YP762233DOT
CSB-EP762233DOT
CSB-BP762233DOT
CSB-MP762233DOT
CSB-EP762233DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nuclear fusion protein KAR5 (KAR5), partial CSB-YP762234DOT1
CSB-EP762234DOT1
CSB-BP762234DOT1
CSB-MP762234DOT1
CSB-EP762234DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA replication complex GINS protein PSF2 (PSF2) CSB-YP762235DOT
CSB-EP762235DOT
CSB-BP762235DOT
CSB-MP762235DOT
CSB-EP762235DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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