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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP760714BAAE1
CSB-EP760714BAAE1
CSB-BP760714BAAE1
CSB-MP760714BAAE1
CSB-EP760714BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UvrABC system protein B (uvrB), partial CSB-YP760715BAAE
CSB-EP760715BAAE
CSB-BP760715BAAE
CSB-MP760715BAAE
CSB-EP760715BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0042 nucleotide-binding protein BT9727_4833 (BT9727_4833) CSB-YP760716BAAE
CSB-EP760716BAAE
CSB-BP760716BAAE
CSB-MP760716BAAE
CSB-EP760716BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian SsrA-binding protein (smpB) CSB-YP760717BAAE
CSB-EP760717BAAE
CSB-BP760717BAAE
CSB-MP760717BAAE
CSB-EP760717BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Tyrosine--tRNA ligase 2 (tyrS2) CSB-YP760718BAAE
CSB-EP760718BAAE
CSB-BP760718BAAE
CSB-MP760718BAAE
CSB-EP760718BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian L-lactate dehydrogenase 2 (ldh2) CSB-YP760719BAAE
CSB-EP760719BAAE
CSB-BP760719BAAE
CSB-MP760719BAAE
CSB-EP760719BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian S-adenosylmethionine synthase (metK) CSB-YP760720BAAE
CSB-EP760720BAAE
CSB-BP760720BAAE
CSB-MP760720BAAE
CSB-EP760720BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S4 (rpsD) CSB-YP760721BAAE
CSB-EP760721BAAE
CSB-BP760721BAAE
CSB-MP760721BAAE
CSB-EP760721BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0173 metal-dependent hydrolase BT9727_4343 (BT9727_4343) CSB-YP760722BAAE
CSB-EP760722BAAE
CSB-BP760722BAAE
CSB-MP760722BAAE
CSB-EP760722BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP760723BAAE
CSB-EP760723BAAE
CSB-BP760723BAAE
CSB-MP760723BAAE
CSB-EP760723BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Asparagine--tRNA ligase (asnS) CSB-YP760724BAAE
CSB-EP760724BAAE
CSB-BP760724BAAE
CSB-MP760724BAAE
CSB-EP760724BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribonuclease PH (rph) CSB-YP760725BAAE
CSB-EP760725BAAE
CSB-BP760725BAAE
CSB-MP760725BAAE
CSB-EP760725BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP760726BAAE
CSB-EP760726BAAE
CSB-BP760726BAAE
CSB-MP760726BAAE
CSB-EP760726BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP760727BAAE
CSB-EP760727BAAE
CSB-BP760727BAAE
CSB-MP760727BAAE
CSB-EP760727BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Uridine kinase (udk) CSB-YP760728BAAE
CSB-EP760728BAAE
CSB-BP760728BAAE
CSB-MP760728BAAE
CSB-EP760728BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP760729BAAE
CSB-EP760729BAAE
CSB-BP760729BAAE
CSB-MP760729BAAE
CSB-EP760729BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosomal protein L11 methyltransferase (prmA) CSB-YP760730BAAE
CSB-EP760730BAAE
CSB-BP760730BAAE
CSB-MP760730BAAE
CSB-EP760730BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative phosphotransferase BT9727_4034 (BT9727_4034) CSB-YP760731BAAE
CSB-EP760731BAAE
CSB-BP760731BAAE
CSB-MP760731BAAE
CSB-EP760731BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphate import ATP-binding protein PstB (pstB) CSB-YP760732BAAE
CSB-EP760732BAAE
CSB-BP760732BAAE
CSB-MP760732BAAE
CSB-EP760732BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP760733BAAE
CSB-EP760733BAAE
CSB-BP760733BAAE
CSB-MP760733BAAE
CSB-EP760733BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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