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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Protochlamydia amoebophila Trigger factor (tig) CSB-YP761034PAAD
CSB-EP761034PAAD
CSB-BP761034PAAD
CSB-MP761034PAAD
CSB-EP761034PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Probable transcriptional regulatory protein pc1328 (pc1328) CSB-YP761035PAAD
CSB-EP761035PAAD
CSB-BP761035PAAD
CSB-MP761035PAAD
CSB-EP761035PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Cysteine--tRNA ligase (cysS) CSB-YP761036PAAD
CSB-EP761036PAAD
CSB-BP761036PAAD
CSB-MP761036PAAD
CSB-EP761036PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP761037PAAD
CSB-EP761037PAAD
CSB-BP761037PAAD
CSB-MP761037PAAD
CSB-EP761037PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribonuclease Y (rny), partial CSB-YP761038PAAD
CSB-EP761038PAAD
CSB-BP761038PAAD
CSB-MP761038PAAD
CSB-EP761038PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Chorismate synthase (aroC) CSB-YP761039PAAD
CSB-EP761039PAAD
CSB-BP761039PAAD
CSB-MP761039PAAD
CSB-EP761039PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP761040PAAD
CSB-EP761040PAAD
CSB-BP761040PAAD
CSB-MP761040PAAD
CSB-EP761040PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Chaperone protein ClpB (clpB), partial CSB-YP761041PAAD
CSB-EP761041PAAD
CSB-BP761041PAAD
CSB-MP761041PAAD
CSB-EP761041PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribonuclease HII (rnhB) CSB-YP761042PAAD
CSB-EP761042PAAD
CSB-BP761042PAAD
CSB-MP761042PAAD
CSB-EP761042PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Peptide chain release factor 1 (prfA) CSB-YP761043PAAD
CSB-EP761043PAAD
CSB-BP761043PAAD
CSB-MP761043PAAD
CSB-EP761043PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP761044PAAD
CSB-EP761044PAAD
CSB-BP761044PAAD
CSB-MP761044PAAD
CSB-EP761044PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L7/L12 (rplL) CSB-YP761045PAAD
CSB-EP761045PAAD
CSB-BP761045PAAD
CSB-MP761045PAAD
CSB-EP761045PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP761046PAAD1
CSB-EP761046PAAD1
CSB-BP761046PAAD1
CSB-MP761046PAAD1
CSB-EP761046PAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP761047PAAD
CSB-EP761047PAAD
CSB-BP761047PAAD
CSB-MP761047PAAD
CSB-EP761047PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S13 (rpsM) CSB-YP761048PAAD
CSB-EP761048PAAD
CSB-BP761048PAAD
CSB-MP761048PAAD
CSB-EP761048PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L14 (rplN) CSB-YP761049PAAD
CSB-EP761049PAAD
CSB-BP761049PAAD
CSB-MP761049PAAD
CSB-EP761049PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 50S ribosomal protein L2 (rplB) CSB-YP761050PAAD
CSB-EP761050PAAD
CSB-BP761050PAAD
CSB-MP761050PAAD
CSB-EP761050PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Asparagine--tRNA ligase (asnS) CSB-YP761051PAAD
CSB-EP761051PAAD
CSB-BP761051PAAD
CSB-MP761051PAAD
CSB-EP761051PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP761052PAAD
CSB-EP761052PAAD
CSB-BP761052PAAD
CSB-MP761052PAAD
CSB-EP761052PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP761053PAAD
CSB-EP761053PAAD
CSB-BP761053PAAD
CSB-MP761053PAAD
CSB-EP761053PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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