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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma mycoides subsp. mycoides SC Glutamate--tRNA ligase (gltX) CSB-YP761114MLS
CSB-EP761114MLS
CSB-BP761114MLS
CSB-MP761114MLS
CSB-EP761114MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC Asparagine--tRNA ligase (asnS) CSB-YP761115MLS
CSB-EP761115MLS
CSB-BP761115MLS
CSB-MP761115MLS
CSB-EP761115MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mycoides subsp. mycoides SC FMN-dependent NADH-azoreductase (azoR) CSB-YP761116MLS
CSB-EP761116MLS
CSB-BP761116MLS
CSB-MP761116MLS
CSB-EP761116MLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Uncharacterized protein B4B2.090 (B4B2.090, NCU08876, NCU10291), partial CSB-YP761117NHA
CSB-EP761117NHA
CSB-BP761117NHA
CSB-MP761117NHA
CSB-EP761117NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE50 (ppe50) CSB-YP761118MVZ
CSB-EP761118MVZ
CSB-BP761118MVZ
CSB-MP761118MVZ
CSB-EP761118MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Follistatin-related protein 4 (FSTL4), partial CSB-YP761119HU
CSB-EP761119HU
CSB-BP761119HU
CSB-MP761119HU
CSB-EP761119HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein ZnFP12 CSB-YP761120HU
CSB-EP761120HU
CSB-BP761120HU
CSB-MP761120HU
CSB-EP761120HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Molybdenum import ATP-binding protein ModC (modC) CSB-YP761121RLU
CSB-EP761121RLU
CSB-BP761121RLU
CSB-MP761121RLU
CSB-EP761121RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Chaperone protein ClpB (clpB), partial CSB-YP761122RLU
CSB-EP761122RLU
CSB-BP761122RLU
CSB-MP761122RLU
CSB-EP761122RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Shikimate dehydrogenase (aroE) CSB-YP761123RLU
CSB-EP761123RLU
CSB-BP761123RLU
CSB-MP761123RLU
CSB-EP761123RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Adenylosuccinate synthetase (purA) CSB-YP761124RLU
CSB-EP761124RLU
CSB-BP761124RLU
CSB-MP761124RLU
CSB-EP761124RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP761125RLU
CSB-EP761125RLU
CSB-BP761125RLU
CSB-MP761125RLU
CSB-EP761125RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L36 (rpmJ) CSB-YP761126RLU
CSB-EP761126RLU
CSB-BP761126RLU
CSB-MP761126RLU
CSB-EP761126RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Bifunctional protein HldE (hldE) CSB-YP761127RLU
CSB-EP761127RLU
CSB-BP761127RLU
CSB-MP761127RLU
CSB-EP761127RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 (ispH1) CSB-YP761128RLU
CSB-EP761128RLU
CSB-BP761128RLU
CSB-MP761128RLU
CSB-EP761128RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP761129RLU
CSB-EP761129RLU
CSB-BP761129RLU
CSB-MP761129RLU
CSB-EP761129RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Elongation factor Tu (tuf1) CSB-YP761130RLU
CSB-EP761130RLU
CSB-BP761130RLU
CSB-MP761130RLU
CSB-EP761130RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S12 (rpsL) CSB-YP761131RLU
CSB-EP761131RLU
CSB-BP761131RLU
CSB-MP761131RLU
CSB-EP761131RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S19 (rpsS) CSB-YP761132RLU
CSB-EP761132RLU
CSB-BP761132RLU
CSB-MP761132RLU
CSB-EP761132RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L5 (rplE) CSB-YP761133RLU
CSB-EP761133RLU
CSB-BP761133RLU
CSB-MP761133RLU
CSB-EP761133RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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