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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodopseudomonas palustris tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP761154RLU
CSB-EP761154RLU
CSB-BP761154RLU
CSB-MP761154RLU
CSB-EP761154RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Uncharacterized RNA methyltransferase RPA0948 (RPA0948) CSB-YP761155RLU
CSB-EP761155RLU
CSB-BP761155RLU
CSB-MP761155RLU
CSB-EP761155RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L34 (rpmH) CSB-YP761156RLU
CSB-EP761156RLU
CSB-BP761156RLU
CSB-MP761156RLU
CSB-EP761156RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP761157RLU
CSB-EP761157RLU
CSB-BP761157RLU
CSB-MP761157RLU
CSB-EP761157RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP761158RLU1
CSB-EP761158RLU1
CSB-BP761158RLU1
CSB-MP761158RLU1
CSB-EP761158RLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP761159RLU
CSB-EP761159RLU
CSB-BP761159RLU
CSB-MP761159RLU
CSB-EP761159RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Translation initiation factor IF-2 (infB), partial CSB-YP761160RLU
CSB-EP761160RLU
CSB-BP761160RLU
CSB-MP761160RLU
CSB-EP761160RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Argininosuccinate synthase (argG) CSB-YP761161RLU
CSB-EP761161RLU
CSB-BP761161RLU
CSB-MP761161RLU
CSB-EP761161RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protein grpE (grpE) CSB-YP761162RLU
CSB-EP761162RLU
CSB-BP761162RLU
CSB-MP761162RLU
CSB-EP761162RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Protein-export protein SecB (secB) CSB-YP761163RLU
CSB-EP761163RLU
CSB-BP761163RLU
CSB-MP761163RLU
CSB-EP761163RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Putative phosphotransferase RPA0298 (RPA0298) CSB-YP761164RLU
CSB-EP761164RLU
CSB-BP761164RLU
CSB-MP761164RLU
CSB-EP761164RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP761165RLU
CSB-EP761165RLU
CSB-BP761165RLU
CSB-MP761165RLU
CSB-EP761165RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP761166RLU
CSB-EP761166RLU
CSB-BP761166RLU
CSB-MP761166RLU
CSB-EP761166RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP synthase subunit delta (atpH) CSB-YP761167RLU
CSB-EP761167RLU
CSB-BP761167RLU
CSB-MP761167RLU
CSB-EP761167RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Gamma-glutamyl phosphate reductase (proA) CSB-YP761168RLU
CSB-EP761168RLU
CSB-BP761168RLU
CSB-MP761168RLU
CSB-EP761168RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP761169RLU
CSB-EP761169RLU
CSB-BP761169RLU
CSB-MP761169RLU
CSB-EP761169RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP761170RLU
CSB-EP761170RLU
CSB-BP761170RLU
CSB-MP761170RLU
CSB-EP761170RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP761171CQF
CSB-EP761171CQF
CSB-BP761171CQF
CSB-MP761171CQF
CSB-EP761171CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (Ile)-lysidine synthase (tilS) CSB-YP761172CQF
CSB-EP761172CQF
CSB-BP761172CQF
CSB-MP761172CQF
CSB-EP761172CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate racemase (murI) CSB-YP761173CQF
CSB-EP761173CQF
CSB-BP761173CQF
CSB-MP761173CQF
CSB-EP761173CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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