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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Sorting nexin-33 (snx33), partial CSB-YP761214XBE
CSB-EP761214XBE
CSB-BP761214XBE
CSB-MP761214XBE
CSB-EP761214XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleolar complex protein 4 homolog A (noc4l-a), partial CSB-YP761215XBE1
CSB-EP761215XBE1
CSB-BP761215XBE1
CSB-MP761215XBE1
CSB-EP761215XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Casein kinase I isoform gamma-1 (csnk1g1) CSB-YP761216XBE
CSB-EP761216XBE
CSB-BP761216XBE
CSB-MP761216XBE
CSB-EP761216XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein Spindly-B (ccdc99-b), partial CSB-YP761217XBE
CSB-EP761217XBE
CSB-BP761217XBE
CSB-MP761217XBE
CSB-EP761217XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Endoplasmic reticulum-Golgi intermediate compartment protein 1 (ergic1), partial CSB-YP761218XBE1
CSB-EP761218XBE1
CSB-BP761218XBE1
CSB-MP761218XBE1
CSB-EP761218XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA oxidative demethylase ALKBH2 (ALKBH2) CSB-YP761219HU
CSB-BP761219HU
CSB-MP761219HU
CSB-EP761219HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse F-box only protein 41 (Fbxo41), partial CSB-YP761220MO
CSB-EP761220MO
CSB-BP761220MO
CSB-MP761220MO
CSB-EP761220MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pleckstrin homology-like domain family B member 3 (PHLDB3) CSB-YP761221HU
CSB-EP761221HU
CSB-BP761221HU
CSB-MP761221HU
CSB-EP761221HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human TMF-regulated nuclear protein 1 (TRNP1) CSB-YP761223HU
CSB-EP761223HU
CSB-BP761223HU
CSB-MP761223HU
CSB-EP761223HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ras-related GTP-binding protein B (Rragb) CSB-YP761224MO
CSB-EP761224MO
CSB-BP761224MO
CSB-MP761224MO
CSB-EP761224MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Iron-responsive element-binding protein 2 (ireb2), partial CSB-YP761225XBE
CSB-EP761225XBE
CSB-BP761225XBE
CSB-MP761225XBE
CSB-EP761225XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Inositol monophosphatase 3 (impad1), partial CSB-YP761226XBE1
CSB-EP761226XBE1
CSB-BP761226XBE1
CSB-MP761226XBE1
CSB-EP761226XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Alpha- (1,3)-fucosyltransferase 10 (fut10), partial CSB-YP761227XBE
CSB-EP761227XBE
CSB-BP761227XBE
CSB-MP761227XBE
CSB-EP761227XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein phosphatase 1 regulatory subunit 26 (ppp1r26), partial CSB-YP761228XBE
CSB-EP761228XBE
CSB-BP761228XBE
CSB-MP761228XBE
CSB-EP761228XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SH3 domain-binding protein 4-A (sh3bp4-a), partial CSB-YP761229XBE
CSB-EP761229XBE
CSB-BP761229XBE
CSB-MP761229XBE
CSB-EP761229XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein kinase pdik1l-A (pdik1-a) CSB-YP761230XBE
CSB-EP761230XBE
CSB-BP761230XBE
CSB-MP761230XBE
CSB-EP761230XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleolar complex protein 4 homolog B (noc4l-b), partial CSB-YP761231XBE1
CSB-EP761231XBE1
CSB-BP761231XBE1
CSB-MP761231XBE1
CSB-EP761231XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Carbonyl reductase family member 4 (cbr4) CSB-YP761232XBE
CSB-EP761232XBE
CSB-BP761232XBE
CSB-MP761232XBE
CSB-EP761232XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nitric oxide synthase-interacting protein (nosip) CSB-YP761233XBE
CSB-EP761233XBE
CSB-BP761233XBE
CSB-MP761233XBE
CSB-EP761233XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human All-trans-retinol 13,14-reductase (RETSAT) CSB-YP761234HU
CSB-EP761234HU
CSB-BP761234HU
CSB-MP761234HU
CSB-EP761234HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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