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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anopheles gambiae Pescadillo homolog (AGAP007112), partial CSB-YP762998BZL
CSB-EP762998BZL
CSB-BP762998BZL
CSB-MP762998BZL
CSB-EP762998BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Casein kinase I (CK1) CSB-YP762999PMT
CSB-EP762999PMT
CSB-BP762999PMT
CSB-MP762999PMT
CSB-EP762999PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calcium-dependent protein kinase 4 (CPK4), partial CSB-YP763000PMT
CSB-EP763000PMT
CSB-BP763000PMT
CSB-MP763000PMT
CSB-EP763000PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant U1 small nuclear ribonucleoprotein C (PY01791) CSB-YP763001PMT
CSB-EP763001PMT
CSB-BP763001PMT
CSB-MP763001PMT
CSB-EP763001PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NACHT, LRR and PYD domains-containing protein 9 (NLRP9), partial CSB-YP763002HU
CSB-EP763002HU
CSB-BP763002HU
CSB-MP763002HU
CSB-EP763002HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine protease 45 (PRSS45) CSB-YP763003HU
CSB-EP763003HU
CSB-BP763003HU
CSB-MP763003HU
CSB-EP763003HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 40S ribosomal protein S5 (rps-5) CSB-YP763004NHA
CSB-EP763004NHA
CSB-BP763004NHA
CSB-MP763004NHA
CSB-EP763004NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Phosphoenolpyruvate carboxykinase [ATP] (pck-1), partial CSB-YP763005NHA
CSB-EP763005NHA
CSB-BP763005NHA
CSB-MP763005NHA
CSB-EP763005NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Survival factor 1 (svf-1) CSB-YP763006NHA
CSB-EP763006NHA
CSB-BP763006NHA
CSB-MP763006NHA
CSB-EP763006NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Peptidyl-prolyl cis-trans isomerase-like 2 (cyp-8), partial CSB-YP763007NHA
CSB-EP763007NHA
CSB-BP763007NHA
CSB-MP763007NHA
CSB-EP763007NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histone H3-like centromeric protein cse-4 (cse-4) CSB-YP763008NHA
CSB-EP763008NHA
CSB-BP763008NHA
CSB-MP763008NHA
CSB-EP763008NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP-dependent rRNA helicase rrp-3 (rrp-3), partial CSB-YP763009NHA
CSB-EP763009NHA
CSB-BP763009NHA
CSB-MP763009NHA
CSB-EP763009NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA-splicing factor cwc-21 (cwc-21) CSB-YP763010NHA
CSB-EP763010NHA
CSB-BP763010NHA
CSB-MP763010NHA
CSB-EP763010NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa rRNA-processing protein efg-1 (efg-1) CSB-YP763011NHA
CSB-EP763011NHA
CSB-BP763011NHA
CSB-MP763011NHA
CSB-EP763011NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Phosphatidylglycerol/phosphatidylinositol transfer protein (npc-2) CSB-YP763012NHA
CSB-EP763012NHA
CSB-BP763012NHA
CSB-MP763012NHA
CSB-EP763012NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Vacuolar protein sorting-associated protein 27 (vps-27), partial CSB-YP763013NHA
CSB-EP763013NHA
CSB-BP763013NHA
CSB-MP763013NHA
CSB-EP763013NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein VTS1 (vts-1), partial CSB-YP763014NHA
CSB-EP763014NHA
CSB-BP763014NHA
CSB-MP763014NHA
CSB-EP763014NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein get-1 (get-1), partial CSB-YP763015NHA1
CSB-EP763015NHA1
CSB-BP763015NHA1
CSB-MP763015NHA1
CSB-EP763015NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mediator of RNA polymerase II transcription subunit 14 (rgr-1), partial CSB-YP763016NHA
CSB-EP763016NHA
CSB-BP763016NHA
CSB-MP763016NHA
CSB-EP763016NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Metacaspase-1A (mca-1) CSB-YP763017NHA
CSB-EP763017NHA
CSB-BP763017NHA
CSB-MP763017NHA
CSB-EP763017NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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