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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis COP9 signalosome complex subunit 8 (csn8) CSB-YP761295XBF
CSB-EP761295XBF
CSB-BP761295XBF
CSB-MP761295XBF
CSB-EP761295XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C15orf26 (C15orf26) CSB-YP761296HU
CSB-EP761296HU
CSB-BP761296HU
CSB-MP761296HU
CSB-EP761296HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse tRNA-specific adenosine deaminase 2 (Adat2) CSB-YP761297MO
CSB-EP761297MO
CSB-BP761297MO
CSB-MP761297MO
CSB-EP761297MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein lifeguard 1 (Grina), partial CSB-YP761298RA1
CSB-EP761298RA1
CSB-BP761298RA1
CSB-MP761298RA1
CSB-EP761298RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat B box and SPRY domain-containing protein (Bspry) CSB-YP761299RA
CSB-EP761299RA
CSB-BP761299RA
CSB-MP761299RA
CSB-EP761299RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein GPR108 (Gpr108), partial CSB-YP761300RA1
CSB-EP761300RA1
CSB-BP761300RA1
CSB-MP761300RA1
CSB-EP761300RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Disabled homolog 2-interacting protein (Dab2ip), partial CSB-YP761301RA
CSB-EP761301RA
CSB-BP761301RA
CSB-MP761301RA
CSB-EP761301RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Adaptin ear-binding coat-associated protein 2 (Necap2) CSB-YP761302RA
CSB-EP761302RA
CSB-BP761302RA
CSB-MP761302RA
CSB-EP761302RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ubiquitin conjugation factor E4 A (Ube4a), partial CSB-YP761303RA
CSB-EP761303RA
CSB-BP761303RA
CSB-MP761303RA
CSB-EP761303RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse MAGUK p55 subfamily member 4 (Mpp4) CSB-YP761304MO
CSB-EP761304MO
CSB-BP761304MO
CSB-MP761304MO
CSB-EP761304MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Eukaryotic translation initiation factor 3 subunit E (eif3e) CSB-YP761305XBF
CSB-EP761305XBF
CSB-BP761305XBF
CSB-MP761305XBF
CSB-EP761305XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acid ceramidase (Asah1) CSB-YP761306RA
CSB-EP761306RA
CSB-BP761306RA
CSB-MP761306RA
CSB-EP761306RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Zinc finger and BTB domain-containing protein 8A.2 (zbtb8a.2) CSB-YP761307XBF
CSB-EP761307XBF
CSB-BP761307XBF
CSB-MP761307XBF
CSB-EP761307XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Eukaryotic translation initiation factor 3 subunit D (eif3d), partial CSB-YP761308XBF
CSB-EP761308XBF
CSB-BP761308XBF
CSB-MP761308XBF
CSB-EP761308XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse High mobility group protein B4 (Hmgb4) CSB-YP761309MO
CSB-EP761309MO
CSB-BP761309MO
CSB-MP761309MO
CSB-EP761309MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lysine-specific demethylase phf2 (phf2), partial CSB-YP761310DIL
CSB-EP761310DIL
CSB-BP761310DIL
CSB-MP761310DIL
CSB-EP761310DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pyridoxal-dependent decarboxylase domain-containing protein 1 (PDXDC1) CSB-YP761311HU
CSB-EP761311HU
CSB-BP761311HU
CSB-MP761311HU
CSB-EP761311HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytochrome b5 domain-containing protein 1 (CYB5D1) CSB-YP761312HU
CSB-EP761312HU
CSB-BP761312HU
CSB-MP761312HU
CSB-EP761312HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Neurexin-3-alpha (Nrxn3), partial CSB-YP761313MO
CSB-EP761313MO
CSB-BP761313MO
CSB-MP761313MO
CSB-EP761313MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse FK506-binding protein 15 (Fkbp15), partial CSB-YP761314MO
CSB-EP761314MO
CSB-BP761314MO
CSB-MP761314MO
CSB-EP761314MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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